NI SEPHAROSE 6FF-色譜介質-耗材-生物在線

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上海基星生物科技有限公司
NI SEPHAROSE 6FF

NI SEPHAROSE 6FF

商家詢價

產品名稱： NI SEPHAROSE 6FF

英文名稱： NI SEPHAROSE 6FF

產品編號： 17-5318-01

產品價格： 0

產品產地： 美國

品牌商標： GE

更新時間： null

使用范圍： null

上海基星生物科技有限公司
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17-5318-01 NI SEPHAROSE 6FF, 25 ML
17-5318-02 NI SEPHAROSE 6FF, 100 ML
17-5318-03 NI SEPHAROSE 6FF, 500 ML
17-5318-06 NI SEPHAROSE 6 FF 5 ML
17-5438-01 MABSELECT SURE 25 ML
17-5438-02 MABSELECT SURE 200 ML
17-5448-01 Capto Blue 25 ml
17-5474-01 MabSelect SuRe LX 25 ML
17-5474-02 MabSelect SuRe LX 200 ML
17-6002-35 IMMUNOPRECIP STARTER PACK
18-1022-29 HB AFFINITY CHROMATOGRAPHY
18-1037-46 ANTIBODY PURIFICATION HB
28-9355-97 Dextrin Sepharose High Perform
28-9355-98 Dextrin Sepharose High Perform
28-9356-00 StrepTactin Sepharose High Per
28-9805-82 MembraneProteinPurificationKit
17-5468-15 HiScreen Capto SP ImpRes
17-5470-15 HiScreen Capto Q ImpRes
17-5474-15 HiScreen MabSelect SuRe LX

Ni Sepharose? 6 Fast Flow - Scale-up Purification

  • Designed for scaling up purification of histidine-tagged* proteins.
  • Suitable for gravity-flow purification and multiwell plate screening.
  • High protein binding capacity.
  • Negligible leakage of Ni2+ ions.
  • Compatible with a wide range of reducing agents, detergents, and other additives

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Ni Sepharose? 6 Fast Flow consists of 90-μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged protein. The ligand density of Ni Sepharose 6 Fast Flow ensures high binding capacity while leakage of Ni2+ ions is negligible, which ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications. The high flow rate property of the Sepharose 6 Fast Flow matrix makes it ideal for scaling up purposes.

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Ni Sepharose 6 Fast Flow is designed for scaling up purification of histidine-tagged proteins, gravity-flow purification, and multiwell plate screening.

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Ni Sepharose? 6 Fast Flow - Scale-up Purification

Technical Information


For more information on these products and the complete range of products for purification of histidine-tagged proteins, see Introduction to Tagged Protein Purification.

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Ni Sepharose 6 Fast Flow is supplied preswollen in 20% ethanol. The medium is also available prepacked in 1-ml and 5-ml HisTrap FF and 20-ml HisPrep FF 16/10 columns, see HisPrep? FF 16/10 Column - Easy Scale-up Purification, 1-ml and 5-ml HisTrap FF crude columns, see HisTrap? FF crude Columns/HisTrap FF crude Kit - Convenient Purification from Unclarified Lysates, His MultiTrap FF 96-well plates, see His MultiTrap 96-Well Filter Plates - High-throughput Screening, and in His GraviTrap columns, see His GraviTrap/His GraviTrap Kit - Gravity-flow Purification.

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IMAC Sepharose 6 Fast Flow, see IMAC Sepharose 6 Fast Flow Media/HiTrap IMAC FF Columns/HiPrep IMAC FF 16/10 Column/HiScreen IMAC FF Column, is the product of choice when charging the medium with different metal ions for optimization of purification protocols.

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* For licensing information, see Legal Info.

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TECHNICAL SPECIFICATIONS
Matrix? Highly crossed-linked 6% agarose, precharged with Ni2+?
Average particle size? 90 μm?
Dynamic binding capacity*? Approx. 40 mg histidine-tagged protein/ml medium?
Metal ion capacity? Approx. 15 μmol Ni2+/ml medium?
Compatibility ?during use? Stable in all commonly used buffers, reducing agents, denaturants, and detergents, see Introduction to Tagged Protein Purification for more information.?
Chemical stability?? For one week at 40°C: 0.01 M HCl, 0.1 M NaOH
For 12 h: 1 M NaOH, 70% acetic acid
30 min tested: 30% 2-propanol
1 h tested: 2% SDS?
pH stability?? 2-14 (short term), 3-12 (long term)?
Storage? 20% ethanol?
Storage temperature? 4°C to 30°C?
*?Protein binding capacity is protein-to protein dependent
??Ni2+-stripped medium

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