• 靶標種屬Rabbit
• 特異性By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG.
• 經測試應用ICC,?IHC-P,?ELISA,?WBmore details
• 偶聯物HRP

性能

• 形式Liquid
• 存放說明Shipped at 4°C. Store at +4°C.
• 存儲溶液Preservative: 0.1% Proclin
  Constituents: 0.2% BSA, PBS
• 濃度1 mg 濃度為 1 mg/ml?
  
• 純度Immunogen affinity purified
• 純化說明Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
• 共軛說明Molar enzyme/ antibody protein ratio is 4:1
• 克隆多克隆
• 同種型IgG
• 研究領域
  • Immunology
  ?
  • Immunoglobulins
  ?
  • Heavy Chain
  ?
  • IgG

  • Secondary antibodies
  ?
  • anti-Rabbit
  ?
  • IgG
  ?
  • Enzyme
  ?
  • HRP

應用

Goat Anti-Rabbit IgG Fc (HRP) 圖像

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG Fc (HRP) (ab97200)

  IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with?ab8227, 3μg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97200,?1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

  The inset negative control image is taken from an identical assay without primary antibody.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Western blot - Goat anti-Rabbit IgG Fc (HRP) secondary antibody (ab97200)
  Ab97200 was used at dilution 1/5000 with the primary antibody?ab90045?in WB. See the review on?ab90045.