組織蛋白酶L抗體
產品名稱： 組織蛋白酶L抗體
英文名稱： VCAM-1
產品編號： 0396
產品價格： null
產品產地： 上海
品牌商標： 雅吉
更新時間： null
使用范圍： WB ELISA IHC-P IHC-F IF
上海雅吉生物科技有限公司
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?中文名稱?組織蛋白酶L抗體?
別? ? 名 Cathepsin L heavy chain; Cathepsin L light chain; CathepsinL; CATL; CTSL; CTSL protein; DKFZp686A18159; FLJ31037; Major excreted protein; MEP; cath-L; CL; CATL1_HUMAN; CathepsinL1; Cathepsin L1,Cathepsin-L1; CTSL 1; CTSL-1,CTS L1,CTS-L1.? ??
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研究領域 激酶和磷酸酶??
抗體來源 Rabbit?
克隆類型 Polyclonal?
交叉反應 Human, Rat,? (predicted: Mouse, )?
產品應用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.?
分 子 量 19/30/37kDa?
細胞定位 細胞漿??
性? ? 狀 Liquid?
濃? ? 度 1mg/ml?
免 疫 原 KLH conjugated synthetic peptide derived from human cathepsin L1 proprotein:71-170/334??
亞? ? 型 IgG?
純化方法 affinity purified by Protein A?
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.?
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.?
PubMed PubMed?
產品介紹 The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene.
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Function:
Important for the overall degradation of proteins in lysosomes.
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Subunit:
Dimer of a heavy and a light chain linked by disulfide bonds.
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Subcellular Location:
Lysosome.
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Similarity:
Belongs to the peptidase C1 family.
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SWISS:
P06797
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Gene ID:
1514
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Database links:
Entrez Gene: 1514 HumanEntrez Gene: 13039 MouseEntrez Gene: 25697 RatOmim: 116880 HumanSwissProt: P07711 HumanSwissProt: P06797 MouseSwissProt: P07154 RatUnigene: 726015 HumanUnigene: 930 MouseUnigene: 1294 Rat
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Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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| 產品圖片 |
 Sample:
A549 Cell (Human) Lysate at 30 ug K562 Cell (Human) Lysate at 30 ug Primary: Anti- Cathepsin L (bs-1508R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19/30/37 kD Observed band size: 40 kD  Protein: line1, rat brain lysates, 30ug; line2, rat liver lysates, 30ug;
Primary: Anti-Cathepsin L (bs-1508R) at 1:200; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 37kD Observed band size : 19kD, 37kD  Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cathepsin Polyclonal Antibody, Unconjugated(bs-1508R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cathepsin L Polyclonal Antibody, Unconjugated(bs-1508R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |