醌氧化還原酶抗體
產品名稱： 醌氧化還原酶抗體
英文名稱： NQO1
產品編號： 2184
產品價格： null
產品產地： 上海
品牌商標： 雅吉
更新時間： null
使用范圍： WB ELISA IHC-P IHC-F IF
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- 地址 : 上海市閔行區元江路5500號第1幢5658室
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- 所在區域 : 上海
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| 中文名稱 | 醌氧化還原酶抗體 |
| 別????名 | Azoreductase; Cytochrome b 5 reductase; DHQU; DIA 4; DIA4; Diaphorase (NADH/NADPH) (cytochrome b 5 reductase); Diaphorase (NADH/NADPH) (cytochrome b-5 reductase); Diaphorase (NADH/NADPH) (cytochrome b-5 reductase); Diaphorase (NADH/NADPH); Diaphorase 4; Dioxin inducible 1; DT diaphorase; DT-diaphorase; DTD; Menadione reductase; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase quinone 1; NAD(P)H menadione oxidoreductase 1 dioxin inducible; NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; NMOR 1; NMOR I; NMOR1; NMORI; NQO 1; NQO1_HUMAN; Phylloquinone reductase; Phylloquinone reductase; QR 1; QR1; Quinone reductase 1; Quinone reductase 1.?? |
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研究領域 腫瘤? 信號轉導? 合成與降解? 新陳代謝? ?
抗體來源 Rabbit?
克隆類型 Polyclonal?
交叉反應 Human, Mouse,? (predicted: Pig, Horse, Rabbit, Guinea Pig, )?
產品應用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.?
分 子 量 31kDa?
細胞定位 細胞漿??
性? ? 狀 Liquid?
濃? ? 度 1mg/ml?
免 疫 原 KLH conjugated synthetic peptide derived from human NQO1:201-274/274??
亞? ? 型 IgG?
純化方法 affinity purified by Protein A?
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.?
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.?
PubMed PubMed?
產品介紹 This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].
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Function:
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
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Subunit:
Homodimer.
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Subcellular Location:
Cytoplasm.
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Similarity:
Belongs to the NAD(P)H dehydrogenase (quinone) family.
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SWISS:
P15559
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Gene ID:
1728
合成與降解（Synthesis and Degradation）
醌氧化還原酶（NQO1）是一種黃素酶，它催化醌雙電子還原反應，以減少氧自由基的產生。經研究發現NQO1可能有抗氧化效應，目前多用于帕金森?。≒D）和部分腫瘤方面的研究。
醌是一種有毒的化合物,能誘發哺乳動物細胞癌變、突變和壞死。
Database links:
Entrez Gene: 610935 DogEntrez Gene: 1728 HumanEntrez Gene: 18104 MouseEntrez Gene: 100286873 PigEntrez Gene: 24314 RatOmim: 125860 HumanSwissProt
| 產品圖片 |
 Sample:
Lane1: Liver(Rat) Lysate at 30 ug Lane2: Brain(Rat) Lysate at 30 ug Primary: Anti-NQO1 (bs-2184R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 31kD Observed band size : 30kD  Sample:
A549(Human) Cell Lysate at 30 ug Primary: Anti-NQO1 (bs-2184R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kD  Protein: A549 lysate at 30ug;
Primary: Anti-NQO1 (bs-2184R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size : 31 kD Observed band size :31 kD  Tissue/cell: mouse colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NQO1 Polyclonal Antibody, Unconjugated(bs-2184R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |