甲基化賴氨酸抗體
產品名稱： 甲基化賴氨酸抗體
英文名稱： Methylated (ε-N) Lysine Antibody
產品編號： ICP0501
產品價格： null
產品產地： 加拿大
品牌商標： ImmuneChem
更新時間： null
使用范圍： ELISA WB IHC
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Catalog # Product Description | ICP0501 The affinity purified rabbit polyclonal anti-methylated lysine antibody was developed using a technique unique to ImmuneChem. The methylated lysine antibodies are affinity purified using N-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The antibodies could be utilized for detection, quantization and isolation of proteins with methylated lysine residues. |  Western blot analysis of the methylated histone from melanoma cells with anti-methylated lysine anti-MeK (ICP0501). Histones were isolated with cold 0.1M HCl extraction followed by: A: Blot with 0.5 μg/mL of anti-MeK (ICP0501) in TBSt and blocked with synthetic methylated BSA 100 μg/mL B: Blot with 0.5 μg/mL of anti-MeK (ICP0501) in TBSt | |
Species | Rabbit | ||
Formulation | 250 μg/mL in Tris-phosphate buffer, 50% glycerol | ||
Immunogen | Methylated KLH conjugates | ||
Purification | The antibody was immunoaffinity purified with epsilon N-methyl lysine on agarose. | ||
Specificity | The antibody recognizes proteins or peptides methylated on lysine residues (mono, di-methyllysine). There are no cross reactions with acetylated proteins. The cross reactivity with tri-methyl lysine has not been tested. | ||
Applications | ELISA; WB; IHC | ||
Scientific Description | MeK is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome C, chromosomal proteins, histones and non-histones as well as neural storage body proteins. It has been suggested that methylation of lysine plays an important role in gene silencing.?[1-3] | ||
Storage & Stability | Store product at -20°C. Expiration date is one year from date of shipping if stored properly. | ||
Product Specific References ? | ? ? ??2.?J. Biol. Chem. 2002.?277:?34655-34657.doi:10.1074/jbc.C200433200 ? ? ? ? 4.?Biochem & Biophys Res. Com.?2014.?451?(2): 229-234. ? ?doi:10.1016/j.bbrc.2014.07.11 ? ? ? ? 6.?Proteomics.?2015.?15?(13):?2166–2176.doi:10.1002/pmic.201400521 本公司還提供以下相關優質產品： |