Anti-β-catenin Rabbit Polyclonal Antibody-抗體-抗體-生物在線
武漢紐斯特生物技術有限公司
Anti-β-catenin Rabbit Polyclonal Antibody

Anti-β-catenin Rabbit Polyclonal Antibody

商家詢價

產品名稱： Anti-β-catenin Rabbit Polyclonal Antibody

英文名稱： β-catenin Antibody

產品編號： 21282

產品價格： null

產品產地： 美國

品牌商標： NewEastBio

更新時間： null

使用范圍： ELISA、WB、 IF、IHC

武漢紐斯特生物技術有限公司
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  • 地址 : 武漢市東湖新技術開發區光谷生物城B3-3棟2-3樓
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  • 所在區域 : 湖北
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β-catenin [21282]
[100 μL]		 ?

Catalog Number:??21282
Gene Symbol:??CTNNB1, CTNNB
Description: ??Anti-β-catenin Rabbit Polyclonal Antibody
Background: ??Beta-catenin (or β-catenin) is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is a subunit of the cadherin protein complex and also acts as an intracellular signal transducer in the Wnt signaling pathway.?Deregulation of beta-catenin signaling is an important event in the genesis of a number of malignancies, such as colon cancer, melanoma, hepatocellular carcinoma, ovarian cancer, endometrial cancer, medulloblastoma pilomatricomas, and prostate cancer.
Immunogen: ???A synthetic peptide from the internal region of β-catenin.
Applications: ??ELISA, WB, IF, IHC
Recommended Dilutions: ??
ELISA: 1:1000-1:5000?
WB: 1:500-1:2000?
IHC: 1:50-1:100
Concentration: ??1 mg/ml
Host: ??Rabbit
Format: ??Liquid
Clonality: ??Polyclonal
Isotype: ??
Purity: ???Purified from serum
Storage buffer:
Preservative:??no
Constituents:??PBS (without Mg2+?and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:??Recognizes β-catenin from vertebrates.
Storage Conditions:???Store at -20°C. Avoid freeze / thaw cycles
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Western blot:??
Western blot analysis of recombinant β-catenin protein. Purified His-tagged β-catenin protein (amino acids 1-76) was blotted with anti- β-catenin rabbit polyclonal antibody (Cat. #21282).
Immunohistochemistry:??
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti-β-catenin polyclonal antibody (Cat. #21282). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.