Size??100ug
Cat code??200333
Species Cross-reactivity??H
Key Application??WB,ICC
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG1
Molecular Weight??140kDa
Sensitivity??This antibody detects endogenous levels USP7 and does not cross-react with related proteins.
Immunogen??Purified recombinant human USP7 protein fragments expressed in E.coli.
UniProt Number??Q93009
GeneBank ID??NM_003470
Subcellular location??Nucleus. Cytoplasm
Formulation??Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername??TEF1; HAUSP
Gene_symbol??TEF1; HAUSP
Summary??Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action. Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD and JAMM enzymes. Herpesvirus-associated ubiquitin-specific protease (HAUSP, USP7) is an important deubiquitinase belonging to USP subfamily. A key HAUSP function is to bind and deubiquitinate the p53 transcription factor and an associated regulator protein Mdm2, thereby stabilizing both proteins. In addition to regulating essential components of the p53 pathway, HAUSP also modifies other ubiquitinylated proteins such as members of the FoxO family of forkhead transcription factors and the mitotic stress checkpoint protein CHFR.
UniPort summary  Function??Hydrolase that deubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, PTEN and DAXX. Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity. In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. Involved in cell proliferation during early embryonic development. Contributes to the overall stabilization and trans-activation capability of the herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 during HSV-1 infection.
Application Images :??
Western blot detection of USP7(C-terminus) antibody in Hela cell lysates using USP7(C-terminus) antibody (1:1000 diluted).
Predicted band size: 129KDa
Observed band size: 140KDa
Immunocytochemistry of HeLa cells using anti- USP7 (C-terminus) antibody diluted 1:150.