sterility:sterile-filtered.
form:liquid.
technique(s):cell culture | insect: suitable.
components:glucose: 1.0 g/L;NaHCO3: 0.35 g/L;L-glutamine: 0.6 g/L.
General description:
TC-100 Insect Medium has been developed to optimize the production of Autographica californica nuclear polyhedrosis virus (NPV) virions by cells from the fall armyworm, Spodoptera frugiperda. It has been found to support the growth of cells derived from a variety of lepidopteran species. The medium is mostly employed in the growth of baculoviruses in lepidopteran cell lines.
Application:
For baculovirus culturing in lepidoptera.
TC-100 Insect Medium has been used:
in the transfection of insect cells in suspension for efficient baculovirus generation
to harvest Varroa destructor saliva
as a starter media to culture and maintain insect cells such as Spodoptera frugiperda