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Disease:

 Basophilic leukemia  

Origin:

 Chemically induced basophil 

Species:

 Rattus norvegicus , Rattus sp., Wistar 

Tissue:

 Peripheral blood 

Morphology:

 Fibroblast 

Properties:

 Adherent 

Medium:

 MEM + 15% FBS 

Subculture:

 1:4 to 1:6 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C 

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:

 I 

Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative


References for PBL-2H3 cells:

1. Kulczycki A Jr., et al. The interaction of IgE with rat basophilic leukemia cells. I. Evidence for specific binding of IgE. J. Exp. Med. 139: 600-616, 1974. 

2. Eccleston E, et al. Basophilic leukaemia in the albino rat and a demonstration of the basopoietin. Nat. New Biol. 244: 73-76, 1973.