Disease:

 Colonic adenocarcinoma 

Origin:

Species:

Homo sapiens 

Tissue:

Colon 

Morphology:

Epithelial 

Properties:

Adherent 

Cytogenic data:

Hypertetraploid 

Patient:

Male, Caucasian, 72 yrs of age 

Medium:

MEM + 20% FBS 

Subculture:

1:4 to 1:6 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C 

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:

 I 

Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Resistant 

Hepatitis B: Negative 

Hepatitis C: Negative 

Protein Expression:
 heat stable enterotoxin (Sta, E. coli); epidermal growth factor (EGF) 
Isoenzymes:
 Me-2, 1; PGM3, 1; PGM1, 1; ES-D, 1; AK-1, 1; GLO-1, 1; G6PD, B 
DNA Profile (STR):
 Amelogenin: X , X CSF1PO: 11 D13S317: 11, 13, 14 D16S539: 12, 13 D5S818: 12, 13 D7S820: 12 THO1: 6 TPOX: 9, 11 vWA: 16, 18 D3S1358: 14, 17 D21S11: 30 D18S51: 12 Penta E: 7 Penta D: 9 D8S1179: 12, 14 FGA: 19 
Related cells by Organ:

Caco-2 cells 

DLD-1 cells 

HCA-7 cells 

HCT-116 cells 

SW480 cells 

HT-29 cells 

LS-180 cells 

SW620 cells 

T84 cells 

SW48 cells 

RKO cells 

SNU-407 cells 

SNU-503 cells 

LS-174T cells 

LoVo cells 

HCT15 cells 


References for Caco-2 cells:

1. Chen, H., Attieh, Z. K., Dang, T., Huang, G., van der Hee, R. M., and Vulpe, C. Decreased hephaestin expression and activity leads to decreased iron efflux from differentiated Caco2 cells. J Cell Biochem, 107: 803-808, 2009. 

2. Cosentino, S., Gravaghi, C., Donetti, E., Donida, B. M., Lombardi, G., Bedoni, M., Fiorilli, A., Tettamanti, G., and Ferraretto, A. Caseinphosphopeptide-induced calcium uptake in human intestinal cell lines HT-29 and Caco2 is correlated to cellular differentiation. J Nutr Biochem, 21: 247-254. 

3. Mangian, H. F. and Tappenden, K. A. Butyrate increases GLUT2 mRNA abundance by initiating transcription in Caco2-BBe cells. JPEN J Parenter Enteral Nutr, 33: 607-617; discussion 617, 2009. 

4. Petruzzelli, M., Groen, A. K., van Erpecum, K. J., Vrins, C., van der Velde, A. E., Portincasa, P., Palasciano, G., van Berge Henegouwen, G. P., Lo Sasso, G., Morgano, A., and Moschetta, A. Micellar lipid composition profoundly affects LXR-dependent cholesterol transport across CaCo2 cells. FEBS Lett, 583: 1274-1280, 2009. 

5. Savini, I., Arnone, R., Catani, M. V., and Avigliano, L. Origanum vulgare induces apoptosis in human colon cancer caco2 cells. Nutr Cancer, 61: 381-389, 2009. 

6. Sun, X., Ge, R., Cai, Z., Sun, H., and He, Q. Y. Iron depletion decreases proliferation and induces apoptosis in a human colonic adenocarcinoma cell line, Caco2. J Inorg Biochem, 103: 1074-1081, 2009.