p38α MAPK (L53F8) Mouse mAb
產品名稱： p38α MAPK (L53F8) Mouse mAb
英文名稱： p38α MAPK (L53F8) Mouse mAb
產品編號： #9228
產品價格： 0
產品產地： 美國
品牌商標： CST
更新時間： 2023-09-19T21:32:30
使用范圍： null
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p38α MAPK (L53F8) Mouse mAb #9228
| No. | Size | ? |
|---|---|---|
| 9228S | 100 ul ( 10 western blots ) | ? |
| 9228 | ? | ? |
| Application | Dilution | Species-Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| W | 1:1000 | Human,?Mouse,?Rat,?Monkey,?Pig,S. cerevisiae | Endogenous | 40 | Mouse IgG1 |
| IF-IC | 1:25 | ||||
| F | 1:200 |
Species cross-reactivity is determined by western blot.
Applications Key:?W=Western Blotting,?IF-IC=Immunofluorescence (Immunocytochemistry),?F=Flow Cytometry
p38α MAP Kinase (L53F8) Mouse mAb detects endogenous levels of total p38α MAPK. This antibody does not cross-react with either JNK/SAPK or p42/44 MAPK or other isoforms of p38.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant p38 MAPK protein.
Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells, using p38α MAPK (L53F8) Mouse mAb.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, using p38α MAPK (L53F8) Mouse mAb (blue) compared to a nonspecifc negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or anisomycin-treated (right), using p38-alpha MAPK (L53F8) Mouse mAb (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
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Background
p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).
SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).
- Rouse, J. et al. (1994)?Cell?78, 1027-1037.
- Han, J. et al. (1994)?Science?265, 808-811.
- Lee, J.C. et al. (1994)?Nature?372, 739-746.
- Freshney, N.W. et al. (1994)?Cell?78, 1039-1049.
- Raingeaud, J. et al. (1995)?J. Biol. Chem.?270, 7420-7426.
- Zervos, A.S. et al. (1995)?Proc. Natl. Acad. Sci. USA?92, 10531-10534.
- Zhao, M. et al. (1999)?Mol. Cell. Biol.?19, 21-30.
- Yang, S.H. et al. (1999)?Mol. Cell. Biol.?19, 4028-4038.
- Cuenda, A. et al. (1995)?FEBS Lett?364, 229-33.
- Kumar, S. et al. (1999)?Biochem Biophys Res Commun?263, 825-31.
Application References
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