IDH2 (R172H) 點突變單克隆抗體-抗體-抗體-生物在線
武漢紐斯特生物技術有限公司
IDH2 (R172H) 點突變單克隆抗體

IDH2 (R172H) 點突變單克隆抗體

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產品名稱： IDH2 (R172H) 點突變單克隆抗體

英文名稱： Anti-IDH2 (R172H) Mouse Monoclonal Antibody

產品編號： 26084

產品價格： null

產品產地： 美國

品牌商標： NewEastBio

更新時間： null

使用范圍： ELISA、WB、 IF、IHC

武漢紐斯特生物技術有限公司
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IDH2 (R172H) [26084]
[100 μL]		 ?

Catalog Number:??26084
Gene Symbol:??IDH2
Description: ??Anti-IDH2 (R172H) Mouse Monoclonal Antibody
Background: ??Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-172 in IDH2 to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.
Immunogen: ??A synthetic peptide from the internal region of human IDH2 which includes the mutation of R172H.
Applications: ??ELISA, WB, IHC, IF
Recommended Dilutions: ??
ELISA: 1:1000-1:5000?
WB: 1:100-1:1000?
IHC: 1:50-1:100?
IF: 1:50-1:100
Concentration: ??1 mg/ml
Host: ??mouse
Format: ??Liquid
Clonality: ??Monoclonal
Isotype: ??IgG
Purity: ??Purified from ascites
Storage buffer:
Preservative:??no
Constituents:??PBS (without Mg2+?and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:??Anti-IDH2(R172H) antibody recognizes IDH2(R172H) of vertebrates.
Storage Conditions:??Store at -20°C. Avoid freeze / thaw cycles.
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Western blot:??
Western blot analysis of recombinant IDH2(R172H) and wild-type IDH2 proteins. Purified his-tagged IDH2(R172H) protein (Cat. # 10201) and wild-type IDH2 protein (Cat. # 10200) were blotted with anti-IDH2(R172H) monoclonal antibody (Cat. # 26084) (left panel). Proteins loading were blotted with anti-IDH2 monoclonal antibody (right panel).
Immunofluorescence:??
Immunofluorescence of cells expressing IDH2 proteins with anti-IDH2 (R172H) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2 (R172H) plasmid (right column), then fixed and stained with anti-IDH2 (R172H) monoclonal antibody (Cat. #26084).
Publications:
1.??Specific monoclonal antibodies against IDH1/2 mutations as diagnostic tools for gliomas
????Brain Tumor Pathology. January 2015, Volume 32, Issue 1, pp 3-11
2.??Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer
????Nature 513, 110–114 (04 September 2014)
3.??Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism
????Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015

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