重組人PD-1蛋白(Fc)
產(chǎn)品名稱： 重組人PD-1蛋白(Fc)
英文名稱： Recombinant Human PD-1 Protein
產(chǎn)品編號(hào)： GMP-TL732
產(chǎn)品價(jià)格：
產(chǎn)品產(chǎn)地： China
品牌商標(biāo)： 同立海源生物
更新時(shí)間： 2023-08-23T17:06:46
使用范圍： null
| 規(guī)格 | 價(jià)格 |
| 100μg | 3000.0 |
- 聯(lián)系人 : 崔女士
- 地址 : 北京市大興區(qū)中關(guān)村科技園區(qū)大興生物醫(yī)藥產(chǎn)業(yè)基地華佗路50號(hào)院13號(hào)樓
- 郵編 : 100085
- 所在區(qū)域 : 北京
- 電話 : 186****3653 點(diǎn)擊查看
- 傳真 : 點(diǎn)擊查看
- 郵箱 : cuilimei@seafrom.cn
重組人PD-1蛋白(Fc)|Recombinant Human PD-1 Protein(Fc)，GMP級(jí)
產(chǎn)品貨號(hào)：GMP-TL732
產(chǎn)品描述（Description）
Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
分子別名（Synonym）
PDCD1、CD279、SLEB2
表達(dá)宿主（Expression host）
HEK293細(xì)胞
來源（Source）
Human
蛋白序列（Protein Sequence）
DNA序列編碼人PD-1（NCBI Reference Sequence: NP_005009.2）表達(dá)帶有Fc標(biāo)簽在C末端
分子量（Molecular Weight）
重組人PD-1蛋白包含380個(gè)氨基酸，預(yù)測(cè)理論分子量為43.4kD
純度（Purity）
≥95%采用SDS-PAGE凝膠和高效液相色譜分析
內(nèi)毒素（Endotoxin）
＜0.1EU/ug
生物活性（Biological Activity）
固定人 PD-L1(His 標(biāo)簽）的濃度是 5μg/mL(100uL/孔) 能夠結(jié)合人 PD-1(Fc 標(biāo)簽)的線性范圍是 0.0625-1ug/mL
穩(wěn)定性（Stability）
凍干的樣本的可以在4℃保存24個(gè)月，溶解后的液體可以于-20℃保存6-12個(gè)月，并且避免反復(fù)凍融
組成（Component）
含有6%甘露醇的無菌PBS（PH7.4）的凍干粉
參考文獻(xiàn)
1. PD-1 Expression on Mycobacterium tuberculosis-Specific CD4 T Cells Is Associated With Bacterial Load in Human Tuberculosis Cheryl L. Day, Deborah A. Abrahams, Rubina Bunjun, Lynnett Stone, Marwou de Kock, Gerhard Walzl, Robert J. Wilkinson, Wendy A. Burgers, Willem A. Hanekom Front Immunol. 2018; 9: 1995. Published online 2018 Aug 31. doi: 10.3389/fimmu.2018.01995
注意：For research and manufacturing use.