Anti-Active Gα13-GTP Mouse Monoclonal Antibody-抗體-抗體-生物在線
武漢紐斯特生物技術有限公司
Anti-Active Gα13-GTP Mouse Monoclonal Antibody

Anti-Active Gα13-GTP Mouse Monoclonal Antibody

商家詢價

產品名稱： Anti-Active Gα13-GTP Mouse Monoclonal Antibody

英文名稱： Anti-Active Gα13-GTP Mouse Monoclonal Antibody

產品編號： 26902

產品價格： null

產品產地： 美國

品牌商標： NewEastbio

更新時間： null

使用范圍： IP, IHC

武漢紐斯特生物技術有限公司
  • 聯系人 :
  • 地址 : 武漢市東湖新技術開發區光谷生物城B3-3棟2-3樓
  • 郵編 : 430074
  • 所在區域 : 湖北
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  • 郵箱 : neweast3@126.com

Active Gα13-GTP [26902]
[30 μL]		 ?

Catalog Number:??26902
Gene Symbol:??Gna13
Description: ??Anti-Active Gα13-GTP Mouse Monoclonal Antibody
Background: ??Heterotrimeric G proteins are essential cellular signal transducers. Gα13 is one of the G proteins that could mediate cell migration and angiogenesis. Other biochemical and physiological functions of Gα13 are being explored.
Immunogen: ??Recombinant full length active Gα13 protein
Applications: ??IP, IHC
Recommended Dilutions: ??1 μg for 1~2 mg total cellular proteins for IP;
???????????????????????????????????????????????????1:100 dilution for IHC
Concentration: ??1 mg/ml
Host: ??mouse
Format: ??Liquid
Clonality: ??Monoclonal
Isotype: ??IgG2b
Purity: ??Purified from ascites
Storage buffer:
Preservative:??no
Constituents:??PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:??Anti-active Gα13 monoclonal antibody recognizes active Gα13 of vertebrates.
Storage Conditions:??Store at -20°C. Avoid freeze / thaw cycles
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Immunoprecipitation/Western blot:??
IP/WB analysis of active Gα13 proteins. Purified Gα13 proteins were treated with GDP (lane 1) or GTPGTPγS (lane 2). After immunoprecipitation with anti-active Gα13-GTP mouse monoclonal antibody (Cat. # 26902), these proteins were separated by SDS/PAGE and western blotted with anti- Gα13 mouse monoclonal antibody (Cat. # 26004).
Immunohistochemistry:??
Staining of MEF cells (treated with PDGF) with anti- Gα13-GTP monoclonal antibody showing the localization of Gα13-GTP in the leading edge of migrating cells.
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Publications:
1.??Resistance to inhibitors of cholinesterase-8A (Ric-8A) is critical for growth factor receptor-induced actin cytoskeletal reorganization
????J Biol Chem. 2011 Sep 2;286(35):31055-61
2.??Agonist activation of the G protein-coupled receptor GPR35 involves transmembrane domain III and is transduced via Galpha and beta-arrestin-2
????Br J Pharmacol. 2011 Feb;162(3):733-48
3.??Gα12/13 inhibition enhances the anti-cancer effect of bortezomib through PSMB5 downregulation
????Carcinogenesis (2010)
4.??Suppression of tumor angiogenesis by Galpha(13) haploinsufficiency
????J Biol Chem. 2009 Oct 2;284(40):27409-15

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