HITRAP Q HP
產(chǎn)品名稱： HITRAP Q HP
英文名稱： HITRAP Q HP
產(chǎn)品編號(hào)： 17-1153-01
產(chǎn)品價(jià)格： 0
產(chǎn)品產(chǎn)地： 美國(guó)
品牌商標(biāo)： GE
更新時(shí)間： null
使用范圍： null
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| 17-1153-01 | HITRAP Q HP, 5 X 1 ML |
| 17-1154-01 | HITRAP Q HP 5X5 ML |
| 17-1351-01 | HITRAP PHENYL HP, 5 X 1ML |
| 17-1353-01 | HITRAP PHENYL FF (LS), 5X1ML |
| 17-1355-01 | HITRAP PHENYL FF (HS),5 X 1ML |
| 17-1357-01 | HITRAP BUTYL FF, 5 X 1 ML |
| 17-1359-01 | HITRAP OCTYL FF, 5 X 1 ML |
| 17-1408-01 | HITRAP DESALTING, 5X5ML |
| 17-5053-01 | HITRAP Q FF, 5 X 1 ML |
| 17-5054-01 | HITRAP SP FF, 5 X 1 ML |
| 17-5055-01 | HITRAP DEAE FF 5 X 1 ML |
| 17-5056-01 | HITRAP CM FF, 5 X 1 ML |
| 17-5079-01 | HITRAP RPROTEIN A FF, 5X1ML |
| 17-5079-02 | HITRAP RPROTEIN A FF, 2 X 1 ML |
| 17-5080-01 | HITRAP RPROTEIN A FF, 1X5ML |
| 17-5080-02 | HITRAP RPROTEIN A FF, 5 X 5 ML |
| 17-5110-01 | HITRAP IGM PURIFICATION 5X1ML |
| 17-5111-01 | HITRAP IGY PURIFICATION 1X5 ML |
| 17-5112-01 | HITRAP STREPTAVIDIN HP5X1 ML |
| 17-5130-01 | GSTRAP FF, 5X1ML |
| 17-5130-02 | GSTRAP FF, 2X1ML |
| 17-5130-05 | GSTRAP FF, 100 X 1 ML |
HiTrap? IEX HP Columns
- Prepacked with Q Sepharose? High Performance or SP Sepharose? High Performance for high resolution purifications on 34-μm beads.
- Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
- Designed for use with a syringe, peristaltic pump and chromatography system such as ?KTA?design.
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HiTrap? IEX HP Columns
Technical Information
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HiTrap? SP HP and HiTrap? Q HP ion exchange columns are fast and convenient to use.
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The ion exchange media packed in HiTrap? ion exchange columns are based on Sepharose? High Performance. The small particle size (34μm) allows fast adsorption and desorption even at high sample loadings and flow rates. SP Sepharose? High Performance is a strong cation exchange medium and Q Sepharose? High Performance is a strong anion exchange medium. Both remain charged and have high loading capacities over broad pH ranges.
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| TECHNICAL SPECIFICATIONS | |
| HiTrap? SP HP and HiTrap? Q HP | |
| Media? | SP Sepharose? High Performance Q Sepharose? High Performance? |
| Column volume? | 1 ml or 5 ml? |
| Max. flow rate? | ? |
| 1-ml column? | 4 ml/min? |
| 5-ml column? | 20 ml/min? |
| Recommended flow rate? | |
| 1-ml column? | 1 ml/min? |
| 5-ml column? | 5 ml/min? |
| Max. back pressure? | 3 bar (43 psi, 0.3 MPa)? |
| Storage? | 20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (SP)? |
| Storage temperature? | 4°C to 30°C? |
| ? | ? |
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Separation of human serum proteins on a 1 ml HiTrap? Q column using a one-step gradient. The separation conditions were optimised for the binding of serum components other than the IgG fraction, which passed through. The result of a subsequent SDS-electrophoresis analysis is shown below.
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SDS -PAGE electrophoresis with PhastSystem? and PhastGel? Gradient 8-25, Coomassie? stained. The separation conditions were optimised for the binding of serum components other than the IgG fraction, which passed through. The result of a subsequent SDS-electrophoresis analysis is shown below.
http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=9391