Anti-HRAS (G13A) Mouse Monoclonal Antibody-抗體-抗體-生物在線
武漢紐斯特生物技術有限公司
Anti-HRAS (G13A) Mouse Monoclonal Antibody

Anti-HRAS (G13A) Mouse Monoclonal Antibody

商家詢價

產品名稱： Anti-HRAS (G13A) Mouse Monoclonal Antibody

英文名稱： Anti-HRAS (G13A) Mouse Monoclonal Antibody

產品編號： 26191

產品價格： null

產品產地： 美國

品牌商標： NewEastbio

更新時間： null

使用范圍： ELISA, WB, IHC

武漢紐斯特生物技術有限公司
  • 聯系人 :
  • 地址 : 武漢市東湖新技術開發區光谷生物城B3-3棟2-3樓
  • 郵編 : 430074
  • 所在區域 : 湖北
  • 電話 : 186****7297 點擊查看
  • 傳真 : 點擊查看
  • 郵箱 : neweast3@126.com

?

Ras (G13A) [26191]
[100ul]		 ?

Catalog Number:??26191
Gene Symbol:??H-Ras; K-Ras; N-Ras
Description: ??Anti-HRAS (G13A) Mouse Monoclonal Antibody
Background: ??Belongs to the small GTPase superfamily. Ras family binds GDP/GTP and possess intrinsic GTPase activity, alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).Located in cell membrane, lipid-anchor, cytoplasmic side and Golgi apparatus membrane.
Immunogen: ??A synthetic peptide from the internal region of HRas which includes the mutation of G13A, human origin.
Applications: ??ELISA, WB, IHC
Recommended Dilutions: ??
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentration: ??1 mg/ml
Host: ??Mouse
Format: ??Liquid
Clonality: ??Monoclonal
Isotype: ??
Purity: ??Purified from ascites
Storage buffer:
Preservative:??no
Constituents:??PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:??Recognizes G13A mutant, but not wild-type H-RAS of vertebrates.
Storage Conditions:?? Store at -20°C. Avoid freeze / thaw cycles
?
Western blot:??
Western blot analysis of recombinant HRas (G13A) and wildtype proteins. Purified His-tagged HRas (G13A) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-HRas (G13A) monoclonal antibody (Cat. #26191).
Immunofluorescence: ??
Immunofluorescence of cells expressing Ras proteins with anti-HRas (G13A) antibody. HEK293T cells were transfected with pCDNA3-GFP-HRas (WT) plasmid (left column) or pCDNA3-GFP-HRas (G13A) plasmid (right column), then fixed and stained with anti-HRas (G13A) monoclonal antibody (Cat. #26191).

?

Publications:
1.??DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
????Gene Therapy 21, 888-896 (October 2014)
2.??Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a