Form of Antibody: Mouse IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability: Store at -20℃/1 year?
Clonality: Monoclonal?
Immunogen: The antiserum was produced against a synthetic peptide GKPIPNPLLGLDST coupled to KLH.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity/Sensitivity: V5-tag mouse monoclonal antibody detects endogenous levels of internal, C-terminal, and N-terminal V5- tagged proteins.
Reactivity: All
Applications: WB 1:5000 IP 1:200 IF: 1:1000
The optimal working dilution should be determined by the end user.
References:?

WB:All lanes : V5-tag mouse monoclonal antibody A: HEK 293 cell lysate transfected without V5-tag protein B: HEK 293 cell lysate transfected with V5 -tag protein C: IP (HEK 293 cell without transfected + anti- V5 mAb 4℃ overnight) D: IP (PBS + anti- V5 mAb 4℃ overnight) E: IP (HEK293 transfected+protein G+anti- V5 mAb 4℃ overnight) F: IP (HEK293 transfected+RIPA+Protein G 4℃ overnight) G: Multi Tag Recombinant protein (expressed in E.coli) TP=Target Protein HC=Heavy chain LC=Light Chain