Product: Anti-Ku (p80) mAb, clone 111
Cat. No.: MC-355 (100 μg)
Background:
The Ku autoantigen is a heterodimer of 70 kDa
(p70) and ~80 kDa (p80) proteins. The p70/p80
dimer is important for the function of a 460 kDa
DNA-dependent protein kinase that
phosphorylates certain transcription factors,
including Sp1, Oct-1, p53 and SV40 large T
antigen in vitro. Ku protein plays a role in cell
signaling, proliferation, DNA repair, replication,
transcriptional activation and apoptosis.
Specificity:
Reacts with the p80 Ku antigen subunit.
Recognizes an epitope between aa 610-705 of
p80.
Species Reactivity:
Human and monkey. Does not react with bovine,
rabbit, mouse or rat.
Cellular Localization:
Nuclear
Positive Control:
HeLa, T47D or BT474 cells.
Ig Isotype:
Mouse IgG1/k
Immunogen:
Human B cell nuclei from plasmacytoid 2p68
cells.
Molecular Weight of Antigen:
80 kDa
Format:
100 μg of monoclonal antibody at 200 μg/mL in
10 mM PBS, pH 7.4, with protein stabilizer and
0.09 sodium azide. Purified from ascites fluid
by ammonium sulfate precipitation.
Storage:
Store at 4°C.
Applications and Suggested Dilutions:

Flow cytometry

Immunofluorescence

Immunoprecipitation: (native & denatured)
Use 2 μg/mg protein lysate. Use Protein G.

Western blot: Use at 0.5-1.0 μg/mL for 2 hrs
at RT.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.