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DIRECTIONS:
1. ????? Remove high-density polyethylene vial holder and foam insert from polycarbonate unit.
DO NOT discard foam insert.
2. ????? add ? 100% isopropyl ? alcohol (250ml) to fill line.DO NOT overfill.
3. ???????? Carefully replace foam insert and vial holder.
4. ???????? Place vials containing sample into holes in vial holder.
5. ???????? Place unit in bottom of -70 ℃ mechanical freezer.leave undisturbed for a minimum of 4 hours.
6. ???????? Remove frozen vials from unit and place in a permanent ,long-term storage freezer (e.g.-130 ℃ or below)
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