Cat. No.: PC-478 (1 mL)
Background:
Alpha Fetoprotein (AFP) is normally synthesized
in? the? liver,? intestinal? tract? and? yolk? sac? of? the
fetus.? Antibody? to? AFP? has? been? shown? to? be
useful? in? detecting? hepatocellular? carcinomas
(HCC) and germ cell neoplasms, especially yolk
sac tumors.
?
Species Reactivity:
Human, pig and sheep. Does not react with dog.
Other species not tested.?
Mol. Wt. of the Antigen:
70 kDa
Immunogen:
Purified alpha fetoprotein
Host:
Rabbit
Format:
1? mL? of? immunoglobulin? fraction? from? rabbit
antiserum. Antibody in 10 mM PBS, pH 7.4, with
protein stabilizer and 0.09% sodium azide.
Cellular Location:
Cytoplasmic
?
Storage:
Store at 4°C.?
?
Positive Control:
HEP-G-2? cells,? fetal? liver? or? hepatocellular
carcinoma.
Applications and Suggested Dilutions:

? Western blotting: Use at a 1:100 dilution? for
2 hours at room temperature.

? Immunohistochemistry:? (formalin/paraffin)
Use at a 1:100-1:200 dilution for 30 minutes
at room temperature.?
No? special? treatment? is? required? for? the
immunohistochemistry? staining? of
formalin/paraffin tissues.
The? optimal? dilution? for? a? specific? application
should be determined by the researcher.
Limitations:
For? in vitro? research? use? only.? Not? for? use? in
diagnostics or in humans.
?
Warranty:
No warranties,? expressed? or? implied,? are made
regarding? the? use? of? this? product.? KAMIYA
BIOMEDICAL COMPANY? is? not? liable? for? any
damage,? personal? injury,? or? economic? loss
caused by this product.