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Size??100ug
Cat code??200515
Species Cross-reactivity??H
Key Application??WB,ICC
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG1
Molecular Weight??102kDa
Sensitivity??This antibody detects endogenous levels of HK1 and does not cross-react with related proteins.
Immunogen??Purified recombinant human HK1 protein fragments expressed in E.coli.
UniProt Number??P19367
GeneBank ID??NM_000188
Subcellular location??Mitochondrion outer membrane.
Formulation??Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol
Altername??HK I,glycolytic enzyme,hexokinase type I
brain form hexokinase
Gene_symbol??HKI; HXK1; HK1-ta; HK1-tb; HK1-tc
Summary??Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq, Apr 2009]
UniPort summary  Function??null
Application Images :??
Western blot detection of HK1 in Hela&Jurkat cell lysates using HK1 antibody (1:10000 diluted).
Predicted band size: 102kDa
Observed band size: 102kDa
Immunocytochemistry stain of Hela using HK1 antibody (1:300).