Anti-His MAB(His標(biāo)簽抗體-鼠單抗)
產(chǎn)品名稱： Anti-His MAB(His標(biāo)簽抗體-鼠單抗)
英文名稱： His-Tag Mouse Monoclonal Antibody (4E6)
產(chǎn)品編號(hào)： ZA004
產(chǎn)品價(jià)格： null
產(chǎn)品產(chǎn)地： 北京
品牌商標(biāo)： 亞太恒信
更新時(shí)間： null
使用范圍：
- 聯(lián)系人 :
- 地址 : 北京市海淀區(qū)上地信息路1號(hào)國(guó)際科技創(chuàng)業(yè)園2號(hào)樓1802
- 郵編 : 100085
- 所在區(qū)域 : 北京
- 電話 : 158****5229 QQ:125***4452 點(diǎn)擊查看
- 傳真 : 點(diǎn)擊查看
- 郵箱 : ythxbio@126.com
His-Tag Mouse Monoclonal Antibody (4E6)
Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. His-tag mouse mAb recognizes His-tag placed at N-terminal, C-terminal, and internal regions of fusion proteins.
ProductName: His-Tag Mouse Monoclonal Antibody
Clone Number: 4E6Isotype: IgG1
Immunogen: A synthetic peptide HHHHHH coupled to KLH.
Tested applications and dilutions
WB 1:5000, IP 1:200, IF 1:1000
Produce anti-HIS antibody affinity column purify HIS recombinant proteins
Form: Liquid
Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Storage: His antibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
A: HEK 293 cell lysate without transfected B: HEK 293 cell lysate transfected with his-tag protein C: IP (PBS + anti-HIS mAb 4℃ overnight) D: IP (HEK293 transfected+protein G+anti-HIS mAb 4℃ overnight)) E: IP (HEK293 transfected+RIPA+Protein G 4℃ overnight) F: Multi Tag Recombinant protein (expressed in E.coli) TP=Target Protein HC=Heavy chain LC=Light Chain
IF analysis of 293T cells transfected with a HIS-tagged protein, using TDYbio HIS-Tag (4E6) Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-HIS)
