Size??100ug
Cat code??200947-6B12
Species Cross-reactivity??H,M,Mk,R,Hm
Key Application??WB,IP,ICC
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??
Isotype??IgG2b
Molecular Weight??36kDa
Sensitivity??This antibody detects endogenous levels of PCNA and does not cross-react with related proteins.
Immunogen??Purified recombinant human PCNA protein fragments expressed in E.coli.
UniProt Number??P12004
GeneBank ID??NM_002592
Subcellular location??Nucleus.
Formulation??ascites
Altername??Cyclin;DNA polymerase delta auxiliary protein;HGCN8729;MGC8367;Mutagen-sensitive 209 protein;OTTHUMP00000030189;OTTHUMP00000030190;PCNA; Pcna/cyclin;PCNA_HUMAN;PCNAR;Polymerase delta accessory protein;Proliferating cell nuclear antigen.
Gene_symbol??
Summary??The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta.The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication.In response to DNA damage,this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.Two transcript variants encoding the same protein have been found for this gene.Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.[provided by RefSeq,Jul 2008]
UniPort summary  Function??Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase′s processibility during elongation of the leading strand.Induces a robust stimulatory effect on the 3′-5′ exonuclease and 3′-phosphodiesterase,but not apurinic-apyrimidinic (AP) endonuclease,APEX2 activities.Has to be loaded onto DNA in order to be able to stimulate APEX2.Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways.Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair:Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases,while ′Lys-63′-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Application Images :??
Western blot detection of PCNA antibody in Hela,3T3,COS7,C6 and CHO-K1 cell lysates using PCNA antibody (1:1000 diluted).Predicted band size:36KDa.Observed band size:36KDa.
Immunocytochemistry staining of HeLa cells using anti-PCNA antibody (dilution 1:100).Fixed in 100% methanol for 2hr at -20℃.
Immunoprecipitation analysis of Hela cell lysates using PCNA antibody.