人非經典Wnt信號通路定量PCR芯片試劑盒
產品名稱： 人非經典Wnt信號通路定量PCR芯片試劑盒
英文名稱： GQH-WAP
產品編號： GK027
產品價格： 0
產品產地： 美國
品牌商標： sciencell
更新時間： 2026-07-03T10:45:27
使用范圍： null
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?人非經典Wnt信號通路定量PCR芯片試劑盒描述：
ScienCells GeneQuery? Human Noncanonical Wnt Signaling Pathways qPCR Array Kit (GQH-WAP) is designed to facilitate gene expression profiling of 88 key genes involved in alternative Wnt pathways.? This kit emphasizes genes involved in planar cell polarity signaling and calcium signal transduction.? Brief examples of how included genes may be grouped according to function are shown below:
- Planar cell polarity:?DAAM1, CELSR1, FAT1, FZD7, VANGL1
- Calcium:?CASR, CALM1, FOSL1, FZD4, NFATC1
- Signaling modulator:?LRP5, LRP6, SPRY1, EFNB, CASR
- Receptors:?WNT2B, WNT4, WNT5A, WNT9A, WNT11
- Target genes:?RHOA, RACGAP1, CREBBP, CFL1, FUZ
GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.?
GeneQuery? Human Noncanonical Wnt Signaling Pathways qPCR Array Plate Layout* (8 controls?in Bold and Italic)
?
| ? |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
ATN1 |
CAPN2 |
CREBBP |
DGKE |
FAT4 |
FZD6 |
ITPR3 |
PFN1 |
RACGAP1 |
SPRY1 |
WNT4 |
β-actin |
|
B |
AXIN1 |
CASR |
CREBZF |
DVL1 |
FLNA |
FZD7 |
LIMK1 |
PLCD1 |
RGS18 |
TLR4 |
WNT5A |
GAPDH |
|
C |
AXIN2 |
CDX2 |
CSNK1E |
DVL2 |
FOSL1 |
GSK3B |
LRP5 |
PLCE1 |
RGS3 |
VANGL1 |
WNT5B |
LDHA |
|
D |
CALM1 |
CELSR1 |
DAAM1 |
DVL3 |
FUZ |
HGS |
LRP6 |
PLCG1 |
RHOA |
VANGL2 |
WNT6 |
NONO |
|
E |
CAMK2A |
CELSR2 |
DCHS1 |
EFNB1 |
FZD2 |
INTU |
MAPK8 |
PRICKLE1 |
ROR1 |
WNT11 |
WNT7A |
PPIH |
|
F |
CAMK2B |
CELSR3 |
DCHS2 |
FAT1 |
FZD3 |
INVS |
NFATC1 |
PRICKLE2 |
ROR2 |
WNT16 |
WNT7B |
GDC |
|
G |
CAMK4 |
CFL1 |
DGKB |
FAT2 |
FZD4 |
ITPR1 |
NFATC2 |
PRKCD |
RYK |
WNT2B |
WNT9A |
PPC |
|
H |
CAPN1 |
CFL2 |
DGKD |
FAT3 |
FZD5 |
ITPR2 |
PCDH8 |
PTK7 |
SIAH2 |
WNT3A |
WNT9B |
NTC |
?
?* gene selection may be updated based on new research and development
| Catalog No. | GK027 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GQH-WAP |
| Size/Quantity | One 96-well plate, one 20ul reaction per well |
| Product Use | GQH-WAP is for research use only. It is not approved for human or animal use or for application in clinical or in vitro diagnostic procedures. |
| Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| Shipping Info | Ambient temperature. |
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