Disease:

Large cell lung carcinoma 

Origin:

Derived from metastatic pleural effusion 

Species:

Homo sapiens 

Tissue:

Lung 

Morphology:

Epithelial 

Properties:

Adherent 

Cytogenic data:

This is a hypotriploid human cell line 

Patient:

Medium:

RPMI1640 + 10% FBS 

Subculture:

1:3 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C 

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:
 I 
Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative 

Isoenzymes:
 AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1-2; Me-2, 1; PGM1, 1; PGM3, 1 
DNA Profile (STR):
 Amelogenin: X,Y; CSF1PO: 11,12; D13S317: 13; D16S539: 9; D5S818: 9,10; D7S820: 9,12; THO1: 9.3; TPOX: 8; vWA: 17 
Mutations in H460 Cells 

Related cells by Organ:

A427 cells 

A549 cells 

Calu3 cells 

H460 cells 


References for H460 cells:

1. Lim, S. J., Choi, M. K., Kim, M. J., and Kim, J. K. Alpha-tocopheryl succinate potentiates the paclitaxel-induced apoptosis through enforced caspase 8 activation in human H460 lung cancer cells. Exp Mol Med, 41: 737-745, 2009. 

2. Pesic, M., Podolski, A., Rakic, L., and Ruzdijic, S. Purine analogs sensitize the multidrug resistant cell line (NCI-H460/R) to doxorubicin and stimulate the cell growth inhibitory effect of verapamil. Invest New Drugs, 28: 482-492. 

3. Liu, H., Li, L., Li, X. Q., Liu, X. J., and Zhen, Y. S. Enediyne lidamycin enhances the effect of epidermal growth factor receptor tyrosine kinase inhibitor, gefitinib, in epidermoid carcinoma A431 cells and lung carcinoma H460 cells. Anticancer Drugs, 20: 41-49, 2009. 

4. Mollah, M. L., Song, J. C., Park, C. H., Lee, G. D., Hong, J. H., Ryoo, Z. Y., Lee, S., Chang, K. T., and Kim, K. S. Anticancer activity and apoptotic effects of Bulnesia sarmienti against human lung cancer H460 cells. Oncol Res, 18: 259-267, 2009. 

5. Borgovan, T., Bellistri, J. P., Slack, K. N., Kopelovich, L., Desai, M., and Joe, A. K. Inhibition of BCL2 expression and activity increases H460 sensitivity to the growth inhibitory effects of polyphenon E. J Exp Ther Oncol, 8: 129-144, 2009. 

6. Pesic, M., Andjelkovic, T., Bankovic, J., Markovic, I. D., Rakic, L., and Ruzdijic, S. Sulfinosine enhances doxorubicin efficacy through synergism and by reversing multidrug resistance in the human non-small cell lung carcinoma cell line (NCI-H460/R). Invest New Drugs, 27: 99-110, 2009.