Anti-ErbB2 Rabbit Polyclonal Antibody-抗體-抗體-生物在線
武漢紐斯特生物技術有限公司
Anti-ErbB2 Rabbit Polyclonal Antibody

Anti-ErbB2 Rabbit Polyclonal Antibody

商家詢價

產品名稱： Anti-ErbB2 Rabbit Polyclonal Antibody

英文名稱： Anti-ErbB2 Rabbit Polyclonal Antibody

產品編號： 21202

產品價格： null

產品產地： 美國

品牌商標： NewEastBio

更新時間： null

使用范圍： ELISA、WB、 IHC

武漢紐斯特生物技術有限公司
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  • 所在區域 : 湖北
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ERBB2 [21202]
[100 μL]		 ?

Catalog Number:??21202
Gene Symbol:??ERBB2/HER2/Neu
Description: ??Anti-ErbB2 Rabbit Polyclonal Antibody
Background: ??The ErbB family is composed of four plasma membrane-bound receptor tyrosine kinases. Amplification or over-expression of the ERBB2 gene occurs in approximately 30% of breast cancers. HER2 is encoded by ERBB2, a known proto-oncogene located at the long arm of human chromosome 17(17q21-q22). HER2 proteins have been shown to form clusters in cell membranes that may play a role in tumorigenesis.
Immunogen: ??A synthetic peptide from the central of ErbB2 protein.
Applications: ??ELISA, WB, IHC
Recommended Dilutions: ??
ELISA: 1:500-1:5000?
WB: 1:200-1:2000?
IHC: 1:20-1:200
Concentration: ??0.2 mg/ml
Host: ??Rabbit
Format: ??Liquid
Clonality: ??Polyclonal
Isotype: ??
Purity: ??Purified from serum
Storage buffer:
Preservative:??no
Constituents:??PBS (without Mg2+?and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:??Recognizes ErbB2 of vertebrates.
Storage Conditions:??Store at -20°C. Avoid freeze / thaw cycles.
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Western blot:??
Western blot analysis of ErbB2 protein. Purified full length His-tagged ErbB2 protein (Cat. #10208) was blotted with anti-ErbB2 rabbit polyclonal antibody (Cat. #21202).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti-ERBB2 polyclonal antibody (Cat. #21202). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4 °C. A HRP-conjugated Goat anti-Rabbit IgG (1:50) was used as secondary antibody.