Background
Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. Cell
Biolabs’ pMXs retroviral vector is based on Moloney murine leukemia virus (MMLV). The vector
provides the viral package signal, transcription and processing elements, and MCS for cloning of a target
gene. The viral env gene, produced by the package cell line, encodes the envelop protein, which
determines the viral infectivity range. Transfection into a package cell line produces high-titer,
replication-incompetent viruses.
Induced pluripotent stem (iPS) cells can be generated from various somatic cells by the retrovirusmediated
transfection of four transcription factors, namely Oct3/4, Sox2, c-Myc, and Klf4. iPS cells are
indistinguishable from ES cells in morphology, proliferation, gene expression, and teratoma formation.
Furthermore, when transplanted into blastocysts, iPS cells can give rise to adult chimeras, which are
competent for germline transmission.