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Interleukin 18 Binding Protein?( IL18BPa ) Antibody
 
 
 
IL18BPa抗體產品信息
 
 
 
 
Immunogen: 
 
 
Recombinant human IL18BP protein (?Catalog#10357-H08H )
 
 
 
 
 
Clone ID:
 
 
 
9A2D4E11
 
 
 
 
 Ig Type:
 
 
Mouse? IgG1
 
 
 
 
緩沖液:
 
0.2 μm filtered solution in PBS with 5% trehalose
 
 
 
Preparation: 
 
 
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human IL18BPa N-terminal domain ( rhIL18BPa; Catalog#10357-H08H; NP_001034748.1; Met 1- Gly 192 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
 
 
 
 
 
IL18BPa抗體使用指南
 
 
 
 
Specificity: 
 
 
Human IL18BP /?IL18BPa?
 
 
 
 
?
 
 
No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
 
 
 
 
Western blot : 
 
This antibody can be used at 1 - 2 ug/mL with the appropriate secondary reagents to detect human IL18BPa in WB. Using a DAB detection system, the detection limit for human IL18BPa is approximately 20ng/lane under non-reducing conditions and reduced conditions.
 
 
 
儲存方法:
 
This antibody can be stored at 2℃-8℃ for 1 month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
 Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
 
 
 
 
 IL18BP Antibody Related Products & Topics
 
 
相關研究領域:
 
Immunology>>Cytokine & Receptor>>Interleukin & Receptor >>IL-1 superfamily and IL-1R/TLR family >>IL-18BP
 
蛋白:
 
 
 
 
Molecule
 
Species
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
 
 
IL-18BP
 
Mouse
 
IL-18BP Protein, Recombinant
 
50206-M08H
 
 
 
IL-18BPa
 
Human
 
IL-18BPa/Fc Protein, Recombinant
 
10357-H03H
 
 
 
IL-18BPa
 
Human
 
IL-18BPa Protein, Recombinant
 
10357-H08H
 
 
 
 
抗體:
 
 
 
 
Molecule
 
Application
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
 
 
Mouse 
 IL-18BP
 
ELISA
 
Rabbit Polyclonal Antibody
 
50206-RP01
 
 
 
Mouse 
 IL-18BP
 
WB, ELISA
 
Rabbit Polyclonal Antibody (Antigen Affinity Purified)
 
50206-RP02
 
 
 
Human 
 IL-18BPa
 
WB
 
Mouse Monoclonal Antibody
 
10357-MM01
 
 
 
Human 
 IL-18BPa
 
ELISA
 
Mouse Monoclonal Antibody
 
10357-MM02
 
 
 
Human 
 IL-18BPa
 
ELISA
 
Rabbit Polyclonal Antibody
 
10357-RP03
 
 
 
Human 
 IL-18BPa
 
WB, ELISA
 
Rabbit Polyclonal Antibody (Antigen Affinity Purified)
 
10357-RP04
 
 
 
 
 
 IL18BPa Antibody Background
 
 
Interleukin 18 binding protein (IL18BP) is a secreted glycoprotein which antagonizes the activity of the proinflammatory cytokine IL18 by binding to IL18 and blocking its biological activity. At least four isoforms differing in affinity for IL18 are produced from alternative splicing, and IL18 BP isoform a contains one immunoglobulin (Ig)-like C2-type domain. IL18BPa binds IL18 with high affinity, prevents the binding of IL18 to its receptor, and inhibits the production of IFN- gamma, IL8, and activationof NF-kappa B, resulting in reduced T- helper type 1 (Th1) immune responses. IL18BPa is constitutively expressed and secreted in mononuclear cells, and its expression can be induced and enhanced through the IFN- gamma-mediated pathway.The identified IFN-γ/IL18BPa negative feedback loop probably plays a key role in the modulation of both innate and adaptive immunity. A severe IL18/IL18BP imbalance is associated with hemophagocytic syndrome (HPS) characterized by an uncontrolled activation of Th1 lymphocytes and macrophages. In addition, IL18BPa is suggested to has a possible antimetastatic benefit, since IL18 plays a significant role in tumor hepatic metastasis.?
 
 
參考文獻
 
 
1.? Aizawa, Y. et al., 1999, FEBS. Lett. 445: 338-342.
 2.? Kim, S.H. et al., 2000. Proc. Natl. Acad. Sci. USA 97: 1190-1195.?
 3.? Veenstra, K.G. et al., 2002, J. Immunol. 168: 2282-2287.?
 4.? Mazodier, K. et al., 2005, Blood. 106: 3483-3489.
 5.? Carrascal, M.T. et al., 2003, Cancer. Res. 63: 491-497.
 
 
 
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