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產品簡介:
flexcell?F5K細胞應力加載系統為二維細胞和三維細胞提供雙軸向應力和單軸向應力，多達4通道，可以同時進行4個不同的牽張拉伸/壓縮形變率對比實驗，加載頻頻率、形變率可以精確調節、操作簡便，是當今科研界最先進細胞應力加載系統。
特色:該系統對二維、三維細胞和組織雙軸向應力或單軸向應力加載，通過StageFlexer顯微應力加載設備,實時觀察細胞、組織在應力作用下的反應。該應力加載系統使用（FlexStop真空隔離閥）可以選擇性地封阻對細胞的應力加載，該應力加載分析系統是國際領先的細胞組織機械力負荷加載強有力的實時分析工具。
該系統與FlexLink壓力加載傳導儀鏈接后，能同時兼備細胞壓力加載功能;flexcell ?FX-5k??flexlink?tension or compression應力加載或壓力加載傳導儀,可同時進行四個不同的實驗程序！ 應力加載傳導儀或壓力加載傳導儀，和FX-5000T應力加載系統或FX-5000C壓力加載系統連接后使用，通過主機控制，四個FlexLink可以同時操作，進行四個不同的實驗程序。
每個FlexLink傳導儀都是一個獨立的控制系統，和FX-5000T應力加載系統或FX-5000C壓力加載系統連接后即可使用。
FlexLink需要和真空泵或壓力源使用
加載原理：通過控制與真空室相連接的真空泵(真空泵和儲變氣壓系統)，使真空室內的負壓產生周期性變化，從而使密封于其上的培養板彈性基底膜發生形變，從而使貼壁生長的細胞受到機械牽張。該系統通過自身研制的FlexSoft??FX-5000?軟件和FX5K??Tension FlexLink 應力加載控制器對應壓力加載大小、作用頻率、作用持續時間進行智能定量調控...
Flexcell FX-5000T Tension SYSTEM（應力加載系統）?
flexcell?F5K細胞應力加載系統為二維細胞和三維細胞提供雙軸向應力或單軸向應力,flexcellint并提供StageFlexer Microscopy Tension Device(FX-5000T實時應力反應觀察顯微設備),
(注釋：A single-well embodiment of the BioFlex??culture plate designed to allow the user to observe signaling responses to strain in real time on a microscope stage. Applies strain to cells in real time on your microscope stage. Consists of a single 35 mm well with a matrix bonded silicone rubber membrane, similar to the membrane in the BioFlex??culture plates. The membrane can be deformed using the Flexcell??FX-5000??or Flex Jr.??Tension systems. )
實時觀察細胞組織在機械應力加載反應，應力加載系統使用（FlexStop_真空隔離閥）可以選擇性地封阻對細胞的應力加載
（注釋:FlexStop真空隔離閥插入BioFlex細胞培養板，Tissue Train細胞培養板，UniFlex細胞培養板培養孔的底部，封阻對該孔內細胞應力加載。
當測試機械加載對生長在同一培養板上的細胞的影響時，使用真空隔離閥提供方便的無應力對照。）
- 計算機控制應力加載系統，為體外培育細胞提供精確，可控制，可重復，靜態或者周期性應力變化。
- 使用真空泵，拉伸培養板底部彈性硅膠模，細胞培養板底部最高伸展度可達到33%。
- 感應各種細胞在應力刺激下生物化學反應，例如：骨骼細胞，肺細胞，心肌細胞，血細胞，皮膚細胞，肌腱細胞，韌帶細胞，軟骨細胞和骨細胞。
- 通過氣體閥門裝置可以自動調節和控制應力。
- 可以和BioFlex，TissueTrain，Uniflex細胞培養板系列一起使用。
- 同一程序中可以同時運行多種頻率，多種振幅和多種波形（圖3）。
- 使用圓柱形或弧矩形應力加載平臺提供雙軸向應力或單軸向應力。
- 不使用加載平臺可以提供梯度雙軸向應力。
- 同時兼容4個獨立FlexLink應力加載傳導儀和/或壓力加載傳導儀，獨立操作四個不同實驗程序。
- 更好控制在超低或超高應力下波形。
FX-5000 Tension系統附件：
- 主機和17寸液晶顯示器
- FlexSoft FX-5000 軟件
- FX5K??Tension FlexLink 應力加載控制器
- 應力加載附件：
- BioFlex一個應力加載基板，四個密封墊片
- BioFlex 一套直徑為25mmBioFlex應力加載平臺
- 4個BioFlex細胞培養板
- 過濾器，濾水器，泵管，潤滑油
多種波形種類：
- 靜態波形
- 正旋波形
- 心動波形
- 三角波形
- 矩形波形
各種特制波形
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A computer-regulated bioreactor that uses vacuum pressure to apply cyclic or static strain to cells cultured on flexible-bottomed culture plates. With this system equibiaxial or uniaxial tension can be applied to cells in 2D and 3D culture. Main features of the system:
- Computer-regulated bioreactor that applies cyclic or static tensile strains to cells cultured in vitro.
- Uses regulated vacuum pressure to deform flexible-bottomed culture plates producing up to 25% substrate elongation.
- Simulate in vivo tissue strains and frequencies in cells from muscle, lung, heart, blood vessels, skin, tendon, ligament, cartilage and bone.
- Contains state-of-the-art digital valve to automatically regulate and maintain pressure for a specified strain regimen.
- Works with BioFlex, Tissue Train, and UniFlex series culture plates.
- Multiple frequency, amplitude and waveform changes can be programmed in one regimen.
- Uses cylindrical or Arctangle Loading Posts to provide equibiaxial or uniaxial strain, respectively.
- Apply gradient biaxial strain (unconstrained distention) by removing Loading Stations.
- Drives up to four independent FlexLink remote compression and/or tension controllers.
- Better control of waveforms at low and high amplitudes.
Tension System
The FX-5000??Tension System is a patented, computerized, pressure-operated instrument that applies a defined controlled, static or variable duration cyclic tension, to cells growing in vitro. This system utilizes regulated vacuum pressure to deform flexible-bottomed culture plates (Figure 1). When used with?BioFlex??culture plates?and cylindrical-shaped loading posts, this system applies an equibiaxial strain to the cells cultured in monolayer on the flexible-bottomed membrane of the BioFlex??culture well. Similarly, when used withUniFlex??culture plates?and an Arctangle??loading post (Figure 2), this system applies a uniaxial strain. This system can apply a gradient biaxial strain of up to 33% in the absence of loading posts.
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Figure 1. Equibiaxial strain application to cells plated in a BioFlex??well.
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Figure 2. UniFlex??culture plate on ArcTangle??Loading Stations?
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The?StageFlexer??allows users to visualize real-time cellular responses to tension. It is a single-well embodiment of a BioFlex??well that fits on a standard upright microscope stage. A?StageFlexer??Membrane?can be deformed
1) freely in the open chamber producing gradient biaxial strain
or 2) across a cylindrical loading post producing equibiaxial strain.
FX-5000T應力加載系統系統相關耗材和配件:
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1、BioFLEX?雙向應力細胞培養板(BioFLEX??CULTURE PLATES)
BioFlex細胞培養板和FX-5000T應力加載系統配套使用，為細胞提供應力加載。
孔板底部是由彈性硅膠模制作而成，柔韌性好，透明度高。
6孔圓形，總生長面積57.75 cm2?(9.62 cm2/孔).(6-well culture plate with total growth surface area of 57.75 cm2)
孔板底部的硅膠模超薄，厚度僅為0.020",(0.0508cm)可以使用標準正立式顯微鏡觀察細胞。(Optically clear for direct viewing of cells with inverted or upright microscopes)
五種不同包被的培養表面：Amino, Collagen (Type I or IV), Elastin, ProNectin (R GD), Laminin (YIGSR).?
Covalently bound matrix surfaces: Amino, Collagen (Type I or IV), Elastin, Pronectin??(RGD), and Laminin (YIGSR).
光學性能佳，自身熒光低。
當與圓柱形加載平臺共同使用時，可以對細胞進行均勻的徑向和圓周應力加載。
Available individually or by the case of 40.
2、UniFlex?單向應力細胞培養板(UniFlex??Culture Plates)
Uniflex細胞培養板和FX-5000T應力加載系統配套使用，為單層細胞提供單軸向應力。
UniFlex細胞培養板和FX-5000T應力加載系統和TissueTrain細胞培養系統配套使用。
準確測量應力---負壓系統曲線，保證應力的準確性和可重復性。
單軸向應力作用于寬0.6英寸x0.952英寸(3.68 cm2)的長方形區域中。
單軸向應力變幅在+/-1.5%.
五種不同包被的培養表面：Amino, Collagen (Type I or IV) Elastin, ProNectin (RGD), Laminin (YIGSR)?
為獲得準確的單軸向應力，必須和弧矩形應力加載平臺配套使用。
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