Recombinant Biotinylated Cynomolgus ALPP Protein,His-Avi Tag
產(chǎn)品名稱： Recombinant Biotinylated Cynomolgus ALPP Protein,His-Avi Tag
英文名稱： Recombinant Biotinylated Cynomolgus ALPP Protein,His-Avi Tag
產(chǎn)品編號(hào)： 93069ES
產(chǎn)品價(jià)格： 詢價(jià)
產(chǎn)品產(chǎn)地： Yeasen
品牌商標(biāo)： Yeasen
更新時(shí)間： 2025-08-21T09:14:30
使用范圍： null
翌圣生物科技（上海）股份有限公司
- 聯(lián)系人 : 李自轉(zhuǎn)
- 地址 : 上海市浦東新區(qū)天雄路166弄一號(hào)樓三層南單元
- 郵編 : 200030
- 所在區(qū)域 : 上海
- 電話 : 139****5640 點(diǎn)擊查看
- 傳真 : 點(diǎn)擊查看
- 郵箱 : lizizhuan@yeasen.com
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產(chǎn)品介紹
PLAP is the abbreviation for Placental alkaline phosphatase, which is an enzyme protein. PLAP is highly expressed in placental tissue, hence its name. The human placental ALP (PLAP) gene was identified on chromosome 2 and possesses 87% similarity with IAP gene. Interestingly, cancer cells can re-express PLAP gene as Regan isoenzyme. In contrast to the other ALP isoenzymes, PLAP is a polymorphic enzyme with up to 18 allelozymes originating from point mutations. As a result of the low catalytic activity in comparison with the other isoenzymes, PLAP is considered to be inefficient due to the neutral pH of placenta.
Furthermore, the structure of PLAP consists of two disulfide bridges: the first one stabilises the PLAP orientation, which is situated near the anchor of the Asp481 Glycosylyphosphatidylinositol (GPI) and the second stiffens the anchor of the carbohydrate chain and is situated close the first part of the glycosylation. There are several roles by PLAP, including the transmission of mother-to-fetus immunoglobulins G (IgG). PLAP stimulates DNA synthesis and fibroblast cell proliferation with insulin in the presence of Zn2+ and Ca2+. It is a synthesiser for a single tissue called syncytiotrophoblast and responsible for transfer of substances such as nutrients and oxygen. PLAP is an essential modulator of foetal growth. PLAP is also a potential marker for various cancers.
產(chǎn)品性質(zhì)
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產(chǎn)品別稱
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PLAP
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種屬
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Cynomolgus
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表達(dá)序列
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Ile 21 - Asn 504
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Accession
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XP_045223825.1
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標(biāo)簽
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C-His-Avi Tag
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表達(dá)系統(tǒng)
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HEK293
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分子量
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The protein has a predicted MW of 57.4 kDa
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純度
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> 95% as determined by SDS-PAGE
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內(nèi)毒素
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< 1.0 EU per μg by the LAL method.
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制劑
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Supplied as a 0.2 μm filtered solution of PBS, pH7.4.
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外觀
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Liquid
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生物活性
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ELISA: Biotinylated Cynomolgus ALPP Protein; His-Avi Tag was immobilized at 1 μg/ml (100 μL/well) on streptavidin precoated. Increasing concentrations of Anti-ALPPL2 mIgG2a antibody were added.
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產(chǎn)品特色
高純度、高活性、低內(nèi)毒素、高批間一致性
應(yīng)用案例
SDS-PAGE
Figure 1. On SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Figure 2. Biotinylated Cynomolgus ALPP Protein; His-Avi Tag was immobilized at 1 μg/ml (100 μL/well) on streptavidin precoated. Increasing concentrations of Anti-ALPPL2 mIgG2a antibody were added.
存儲(chǔ)條件
-85 ~ -65℃保存，收到貨之日起，6個(gè)月有效期。避免反復(fù)凍融，建議等分分裝為一次實(shí)驗(yàn)使用量，分裝后，-80℃凍存。