Product Highlight

• Configuration-specific monoclonal antibody that recognizes Gα?₁₃-GTP, but not Gα?₁₃-GDP
• The only available Gα?₁₃?activation assay kit directly measures the level of Gα?₁₃-GTP
• Providing reliable results with consistent reproducibility than other Gα?₁₃?activation assay kits based on certain downstream signaling pathways
• Providing shorter experimental time
• Could be used to monitor the activation of Gα?₁₃?in cells and in tissues by immunohistochemistry

Product Description

A structurally diverse repertoire of ligands, from photons to large peptides, activates GPCRs to elicit their physiological functions. Ligand-bound GPCRs, in turn, function as guanine nucleotide exchange factors catalyzing the exchange of GDP bound on the Gα subunit with GTP in the presence of Gβγ, causing the dissociation of the Gα subunit from the Gβγ dimer to form two functional units (α and Gβγ). Both Gα and Gβγ subunits signal to various cellular signaling pathways. Based on the sequence and functional homologies, G proteins are grouped into four families: G?_s, G?_i, G?_q, and G?₁₂. As increasing numbers of effectors and interacting proteins for these G proteins have been identified, the physiological processes in which G proteins participate are multiplying.

Among the four subfamilies of G proteins, the function of G?_12/13?subfamily is less well understood. In this family, there are two members, G?₁₂?and G?₁₃, that are expressed ubiquitously. Gα?₁₂?knockout mice appeared normal. Gα?₁₃?knockout mice displayed embryonic lethality (~E9.5). The Gα?₁₃-/- mouse embryos had defective vascular systems. G?₁₃?is also essential for receptor tyrosine kinase-induced migration of fibroblast and endothelial cells.

NewEast Biosciences Gα?₁₃?Activation Assay Kit provides a simple and fast tool to monitor the activation of Gα?₁₃. Each kit provides sufficient quantities to perform 20 assays.

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Assay Principle

NewEast Biosciences Gα?₁₃?Activation Assay Kit bases on the configuration-specific anti-Gα?₁₃-GTP monoclonal antibody to measure the active Gα?₁₃-GTP levels, either from cell extracts or from in vitro GTPgamma;S loading Gα?₁₃?activation assays. Briefly, anti-active Gα?₁₃?mouse monoclonal antibody will be incubated with cell lysates containing Gα?₁₃-GTP. The bound active Gα?₁₃?will then be pulled down by protein A/G agarose. The precipitated active Gα?₁₃?will be detected by immunoblot analysis using anti-Gα?₁₃?rabbit polyclonal antibody.