Benefits of the CatchPoint cyclic-GMP Assay Kit

·?????????????? High affinity reagents are optimized for sensitivity when cGMP levels are low, ensuring results when other assays fail.

·?????????????? Robust format resists interference from colored or fluorescent compounds, improving reliability of results.

·?????????????? Rapid signal development (< 10 min) and no termination step give users a flexible read time that is compatible with automation and high-throughput screening.

·?????????????? Quantitative method allows accurate determination of cGMP in samples.

CatchPoint cGMP Assay principle. The cGMP in the sample or standard competes with horseradish peroxidase (HRP)-labeled cGMP conjugate for binding sites on the anti-cGMP antibodies. In the absence of cGMP, most of the HRP-cGMP conjugate is bound to the antibody. Increasing concentrations of cGMP competitively decrease the amount of bound conjugate, thus decreasing measured HRP activity.

CatchPoint cyclic-GMP Assay Kit is available in 96- and 384-well formats.? They are also available in CatchPoint cGMP Explorer or Bulk quantities, to meet the throughput needs of different users.

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CatchPoint cGMP Explorer Fluorescent Assay Kit

·?????????????? One CatchPoint cGMP Explorer Kit provides the following components:

·?????????????? Two black clear-bottom plates (96- or 384-well format depending upon specific product)

·?????????????? 100 mL ELISA Assay Buffer

·?????????????? 1 (96-well) or 2 (384-well) vials Rabbit Anti-cGMP Antibody

·?????????????? 1 (96-well) or 2 (384-well) vials cGMP Calibrator

·?????????????? 1 (96-well) or 2 (384-well) vials HRP-cGMP Conjugate

·?????????????? 100mL, 10X Wash Solution Concentrate

·?????????????? 50 mL Cell Lysis Buffer

·?????????????? 1 vial 100X Substrate

·?????????????? 100 mL Substrate Buffer

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CatchPoint cGMP Bulk Fluorescent Assay Kit

·?????????????? One CatchPoint cGMP Bulk Kit provides:

·?????????????? Ten (96-well) or twenty (384-well) black clear-bottom plates

·?????????????? 950 mL (96-well) or 2 x 950 mL (384-well) ELISA Assay Buffer [double-check; amount for 96-well kit is wrong on current webpage]

·?????????????? 2 vials Rabbit Anti-cAMP Antibody

·?????????????? 2 vials cAMP Calibrator

·?????????????? 2 vials HRP-cAMP Conjugate

·?????????????? 950mL, 10X Wash Solution Concentrate

·?????????????? 450 mL (96-well) or 2 x 450 mL Cell Lysis Buffer

·?????????????? 2 x 900 μL (96-well) or 5 x 900 mL (384-well) 100X Substrate

·?????????????? 950 mL Substrate Buffer