Origin:

 Kidney fibroblasts 

Species:

 Cercopithecus aethiops(African Green Monkey) 

Tissue:

 Kidney 

Morphology:

 Fibroblast-like 

Properties:

 Adherent 

Cytogenic data:

 SV40 immortalized 

Medium:

 DMEM + 10% FBS 

Subculture:

 1:4 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 °C 

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:

I 

Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative


References for COS-7 cells:

1. Liu, K., Luo, Y. J., Liu, Y. B., Yao, J., Yang, H., Mou, H., Huang, G. Y., and Zhang, Y. [Construction of eukaryotic expressing vector of multiple myeloma mucin-1 and its expression in COS-7 cells in vitro]. Zhongguo Shi Yan Xue Ye Xue Za Zhi, 17: 898-902, 2009. 

2. Nelson, S. R., Ali, M. Y., Trybus, K. M., and Warshaw, D. M. Random walk of processive, quantum dot-labeled myosin Va molecules within the actin cortex of COS-7 cells. Biophys J, 97: 509-518, 2009. 

3. Kabakov, A. Y. and Rosenberg, P. A. Evidence for change in current-flux coupling of GLT1 at high glutamate concentrations in rat primary forebrain neurons and GLT1a-expressing COS-7 cells. Eur J Neurosci, 30: 186-195, 2009. 

4. Li, L., Nie, Y., Ye, D., and Cai, G. An easy protocol for on-chip transfection of COS-7 cells with a cationic lipid-based reagent. Lab Chip, 9: 2230-2233, 2009. 

5. Yonamine, C. M., Prieto-da-Silva, A. R., Magalhaes, G. S., Radis-Baptista, G., Morganti, L., Ambiel, F. C., Chura-Chambi, R. M., Yamane, T., and Camillo, M. A. Cloning of serine protease cDNAs from Crotalus durissus terrificus venom gland and expression of a functional Gyroxin homologue in COS-7 cells. Toxicon, 54: 110-120, 2009. 

6. Hansen, J. L., Hansen, J. T., Speerschneider, T., Lyngso, C., Erikstrup, N., Burstein, E. S., Weiner, D. M., Walther, T., Makita, N., Iiri, T., Merten, N., Kostenis, E., and Sheikh, S. P. Lack of evidence for AT1R/B2R heterodimerization in COS-7, HEK293, and NIH3T3 cells: how common is the AT1R/B2R heterodimer? J Biol Chem, 284: 1831-1839, 2009. 

7. Nakao, M., Hironaka, S., Harada, N., Adachi, T., Bito, T., Yabuta, Y., Watanabe, F., Miura, T., Yamaji, R., Inui, H., and Nakano, Y. Cobalamin deficiency results in an abnormal increase in L-methylmalonyl-co-enzyme-A mutase expression in rat liver and COS-7 cells. Br J Nutr, 101: 492-498, 2009.