Anti-EGFR (A839T) Mouse Monoclonal Antibody-抗體-抗體-生物在線

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武漢紐斯特生物技術(shù)有限公司
Anti-EGFR (A839T) Mouse Monoclonal Antibody

Anti-EGFR (A839T) Mouse Monoclonal Antibody

商家詢價(jià)

產(chǎn)品名稱： Anti-EGFR (A839T) Mouse Monoclonal Antibody

英文名稱： Anti-EGFR (A839T) Mouse Monoclonal Antibody

產(chǎn)品編號(hào)： 26117

產(chǎn)品價(jià)格： null

產(chǎn)品產(chǎn)地： 美國(guó)

品牌商標(biāo)： NewEastBio

更新時(shí)間： null

使用范圍： ELISA、WB、 IF、IHC

武漢紐斯特生物技術(shù)有限公司
  • 聯(lián)系人 :
  • 地址 : 武漢市東湖新技術(shù)開發(fā)區(qū)光谷生物城B3-3棟2-3樓
  • 郵編 : 430074
  • 所在區(qū)域 : 湖北
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  • 郵箱 : neweast3@126.com

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EGFR (A839T) [26117]
[100 μL]		 ?

Catalog Number:??26117
Gene Symbol:??EGFR/HER1/ERBB1
Description: ??Anti-EGFR (A839T) Mouse Monoclonal Antibody
Background: ??EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
Immunogen: ??A synthetic peptide from the internal region of EGFR which includes the mutation of A839T, human origin.
Tested Applications: ??ELISA, WB, IF, IHC
Recommended Dilutions: ???
ELISA: 1:1000-1:5000?
WB: 1:500-1:1000?
IHC: 1:50-1:100
Concentration: ??1 mg/ml
Host: ??Mouse
Format: ??Liquid
Clonality: ??Monoclonal
Isotype: ??
Purity: ??Purified from ascites
Storage buffer:
Preservative:??no
Constituents:??PBS (without Mg?2+?and Ca?2+?), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:??Recognizes EGFR (A839T), but not wild type EGFR of vertebrates.
Storage Conditions:??Store at -20°C. Avoid freeze / thaw cycles.
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Western blot:??
Western blot analysis of recombinant EGFR(A839T) and wildtype proteins. Purified His-tagged EGFR (A839T) protein (702-987) (Cat. #10221) and wild-type protein (702-987) (Cat. #10210) were blotted with anti- EGFR (A839T) mouse antibody (Cat. #26117).
Immunofluorescence of cells expressing EGFR proteins with anti-EGFR (A839T) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR (A839T) plasmid (right column), then fixed and stained with anti- EGFR (A839T) monoclonal antibody (Cat. #26117).
Publications:
1.??Immunohistochemistry with a novel mutation-specific monoclonal antibody as a screening tool for the EGFR L858R mutational status in primary lung adenocarcinoma
????Tumor Biology. October 2014

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