LightCycler ? Multiplex DNA Master???雙雜交探針?多重DNA大師

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Easy-to-use 5x reaction mix optimized for multiplex qPCR, compatible with the LightCycler? 480 or the LightCycler? 96 Real-Time PCR Systems.

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易于使用的5倍反應混合優化的多重qPCR，與LightCycler?480或LightCycler?96實時PCR系統兼容。

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For general laboratory use.

供一般實驗室使用。

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Product No：07339577001???產品編號：07339577001 ?

Pack Size：1000?x 20 μL?reactions ?包裝尺寸：1000?x 20?μL?反應

Product No：07339585001 ?產品編號：07339585001 ??

Pack Size：200 x 20 μL?reactions ??包裝尺寸：200 x 20 μL 反應

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Keywords: Pathogen Detection??關鍵詞：病原體檢測

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Overview概述：

Rely on this new Real-Time PCR Master Mix for fast and highly sensitive multiplex detection of DNA targets and internal controls. Based on the aptamer-mediated hot start AptaTaq Fast DNA Polymerase and a 5x concentrated buffer formulation optimized for multiplex target detection, enabling sensitive qPCR of pathogen DNA targets isolated from a variety of human sample materials.

依靠這種新型 Real-Time PCR Master Mix 對 DNA 靶標和內部對照進行快速、高靈敏度的多重檢測。基于適體介導的熱啟動 AptaTaq 快速 DNA 聚合酶和針對多重目標檢測優化的 5 倍濃縮緩沖液配方，能夠對從各種人類樣本材料中分離的病原體 DNA 目標進行靈敏的 qPCR。

Perform fast, highly sensitive and specific qPCR detection of pathogen DNA targets isolated from a wide range of materials, including difficult sample materials (e.g., blood, plasma, serum, stool).

對從各種材料中分離的病原體 DNA 靶標進行快速、高靈敏度和特異性 qPCR 檢測，包括難處理的樣品材料（例如，血液、血漿、血清、糞便）.

Increase throughput and cost efficiency of the qPCR workflow with a master mix optimized for multiplex detection of up to 5 targets.

使用針對多達 5 個目標的多重檢測而優化的預混液，提高 qPCR 工作流程的通量和成本效率.

Detect low copy number of DNA targets with higher sensitivity with a 5x master mix concentration

使用 5 倍預混液濃度以更高的靈敏度檢測低拷貝數的 DNA 靶標

Decrease experiment set up time and effort with convenient single vial mix

使用方便的單瓶混合減少實驗設置時間和精力

Automate your workflow or setup your reactions at room temperature with the thermostable enzyme in the master mix

使用預混液中的熱穩定酶自動化您的工作流程或在室溫下設置您的反應

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Contents?內容

qPCR Reaction Mix, 5x conc.

qPCR 反應混合物，5x 濃度。

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Water, PCR grade

水，PCR 級

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Application應用：

The LightCycler? Multiplex DNA Master is a 5x master mix especially suited for the fast and efficient multiplex PCR detection of up to 5 DNA pathogen targets in human sample materials. The robust formulation allows multiplex qPCR detection of a multitude of pathogen targets without the need for reaction condition adaptation or titration of MgCl2 concentration. The PCR protocol has been designed to run on the LightCycler? 480 and LightCycler? 96 Systems. The master mix is optimized for use with hydrolysis probes as well as Universal ProbeLibrary (UPL) probes.

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LightCycler ? ?Multiplex DNA Master 是一種 5倍預混液，特別適用于對人類樣品材料中多達 5 個 DNA 病原體靶標進行快速高效的多重 PCR 檢測。穩健的配方允許多重 qPCR 檢測多種病原體目標，而無需調整反應條件或滴定 MgCl 2 濃度。PCR 方案設計用于在 LightCycler ? ?480 和 LightCycler ? ?96 系統上運行。預混液經過優化，可與水解探針以及 Universal ProbeLibrary (UPL) 探針配合使用。