• 靶標種屬Mouse
• 特異性By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
• 經測試應用ICC,?IHC-P,?ELISA,?WBmore details
• 偶聯物HRP

性能

• 形式Liquid
• 存放說明Shipped at 4°C. Store at +4°C.
• 存儲溶液Preservative: 0.1% Proclin
  Constituents: 0.2% BSA, PBS
• 濃度1 mg 濃度為 1 mg/ml?
  
• 純度Immunogen affinity purified
• 純化說明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
• 共軛說明Molar enzyme/ antibody protein ratio is 4:1
• 克隆多克隆
• 同種型IgG
• 常規說明Part of the?AbExcel?range.
• 研究領域
  • Immunology
  ?
  • Immunoglobulins
  ?
  • Heavy Chain
  ?
  • IgG

  • Secondary antibodies
  ?
  • anti-Mouse
  ?
  • IgG
  ?
  • Enzyme
  ?
  • HRP

應用

Rabbit Anti-Mouse IgG H&L (HRP) 圖像

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046)

  IHC image of?beta actin?staining in?human colon?formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with?ab8224, 3μg/ml overnight at +4°C. An HRP-conjugated secondary (ab97046,?1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

  The inset negative control image is taken from an identical assay without primary antibody.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Western blot - Rabbit anti-Mouse IgG H&L (HRP) secondary antibody (ab97046)

  All lanes :?Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 μg/ml Lanes 1-6 :?HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 μg per lane. Secondary Lanes 1 - 2 :?Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97046) at 1/2000 dilution Lanes 3 - 4 :?Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97046) at 1/10000 dilution Lanes 5 - 6 :?Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97046) at 1/20000 dilution Developed using the

• 

  Western blot - Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97046)
  
  developed using the ECL technique
  
  Performed under non-reducing conditions.
  
  Exposure time :?2 seconds

  Sample: Human Cell lysate - whole cell (human glioma cells), Loading amount: 20 μg, Specification: human glioma cells

  Blocking step: Milk as blocking agent for 1 hour, Concentration: 5 %, Temperature: 25°C

  Other product details: 1st Ab Dilution 1/5000 anti-actin Ab, Incubation time: 16 hours, Temperature: 4°C

  Secondary antibody: ab97046 used at 1:20,000

  Bands: Specific: 45 kDa

  Notes: primary Ab (anti-actin) was used as a loading control in 6 human glioma cell lines

  This image is courtesy of an anonymous Abreview