LightCycler? Fluorescein CPG???

LightCycler ? 熒光素 CPG

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3 Dye when labeling HybProbe probes for single and multicolor detection on all LightCycler? 480 and LightCycler? Carousel-Based System Instruments.

在所有 LightCycler ? ?480 和 LightCycler ?轉盤式 系統儀器上標記用于單色和多色檢測的 HybProbe 探針時的 3 染料。

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For life science research only. Not for use in diagnostic procedures.

僅用于生命科學研究。不用于診斷過程。

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Product No：03138178001???產品編號：03138178001 ?

Pack Size：1g???包裝尺寸：1克

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Keywords: Other qPCR Consumables??關鍵詞：其他 qPCR 耗材

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Overview概述：

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Fluorescein long chain alkylamino Controlled Pore Glass (lcaa CPG, 500 ?) for labeling the 3 terminus of oligonucleotides.

用于標記寡核苷酸 3 末端的熒光素長鏈烷基氨基控制孔玻璃（lcaa CPG，500 ?）。

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Contents?內容

white solid

白色固體

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Application應用：

LightCycler? Fluorescein CPG labels the 3 end of an oligonucleotide that is combined with a 5 LightCycler? Red-laeled oligonucleotide when using HybProbe probes for detection. This support can be used in the same manner as standard nucleoside supports since it contains a dimethobxytrityl group.

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當使用 HybProbe 探針進行檢測時，LightCycler ? 熒光素 CPG 標記寡核苷酸的 3 末端，該寡核苷酸與 5 LightCycler ? ?Red-laeled 寡核苷酸結合。該支持物可以以與標準核苷支持物相同的方式使用，因為它含有二甲氧基三苯甲基。

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Detail 細節

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The LightCycler? Fluorescein CPG is derived from a pure dye isomer, and is attached via a linkage that is stable during oligonucleotide synthesis. Therefore, HPLC purification is simple and results in a well-defined product. HybProbe probes synthesized using the LightCycler? Fluorescein CPG are blocked at the 3 terminus, which prevents them from Taq DNA polymerase extension and exonuclease digestion. Thus, the resulting oligonucleotide is perfectly suited for mono- and dual-color experiments using the LightCycler? Carousel-Based System Instruments.

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LightCycler ? 熒光素 CPG 源自純染料異構體，并通過在寡核苷酸合成過程中穩定的連接鍵連接。因此，HPLC 純化很簡單，可以得到明確定義的產品。使用 LightCycler ? 熒光素 CPG合成的 HybProbe 探針在 3 末端被封閉，從而阻止它們進行 Taq DNA 聚合酶延伸和核酸外切酶消化。因此，所得寡核苷酸非常適合使用 LightCycler ? 基于旋轉木馬的系統儀器進行單色和雙色實驗。

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Spectral characteristics for an oligonucleotide labeled with LightCycler? Fluorescein:

Excitation maximum: 494 nm

Emission maximum: 519 nm (both measured in 2 mM Tris buffer, pH 8.3).

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用 LightCycler ? 熒光素標記的寡核苷酸的光譜特征：

最大激發波長：494 nm

發射最大值：519 nm（均在 2 mM Tris 緩沖液，pH 8.3 中測量）。