Reagent :PEIpro.

Concentration :1 mg/mL.

Molecule delivered :Plasmid DNA and co-delivery of several plasmid DNA.

Applications :Large-scale virus production.

Virus types :Mainly adenovirus, Adeno-Associated Viruses (AAV), Lentivirus, influenza virus, retrovirus, Virus-like particles (VLP).

Cell types :Mammalian producer cell lines (HEK-293, HEK-293 derivatives, BHK, VERO cell lines, virus-specific packaging cell lines.

Number of transfections :100 mL of PEIpro transfection reagent is sufficient to transfect on average 50 L of cell culture.

Storage :
Store PEIpro at 5 °C ± 3°C.
Expiry date is indicated in the certificate of analysis (available in "My account") and on the product.

Provided with :
Protocol.
Certificate of Analysis.
Certificate of Origin.
Non-Hazardous Product Statement.

Summary:PEIpro transfection reagent is the leading chemical-based DNA transfection reagent that offers flexibility and scalability to develop a robust and sustainable Process Development for viral vector production. PEIpro benefits from extensive research development in PEI polymer chemistry and formulation to achieve highest transfection efficiency in both adherent and suspension cell culture systems. Protocols established with PEIpro during Process Development are directly implementable for large-scale manufacturing of preclinical and clinical batches of viral vectors with the higher quality grades of PEIpro: PEIpro-HQ and PEIpro-GMP.