RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE
產(chǎn)品名稱： RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE
英文名稱： RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE
產(chǎn)品編號(hào)： AM1975
產(chǎn)品價(jià)格： 0
產(chǎn)品產(chǎn)地： 美國
品牌商標(biāo)： Invitrogen
更新時(shí)間： 2023-09-19T21:32:30
使用范圍： null
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描述
The RecoverAll? Total Nucleic Acid Isolation Kit for FFPE is for the extraction of total nucleic acid from formalin-
or paraformalin-fixed, paraffin-embedded (FFPE) tissues. Sufficient reagents are included for 40 purifications from up
?to four 20 μm sections, or up to 35 mg of unsectioned, core samples each. Features of the RecoverAll? Total Nucleic
Acid Isolation Kit for FFPE:
? Optimized for isolation of total nucleic acids, including microRNAs, from FFPE tissue
? No overnight Proteinase K digestion required—deparaffinize in the morning and perform qRT-PCR in the afternoon
? Typical yields are >50% of those of unfixed tissue
? Recovered nucleic acids are suitable for real-time RT-PCR, PCR, mutation screening, and microarray analyses
Extraction of nucleic acids from difficult samples
Archived tissue samples contain valuable information of disease states, but it has traditionally been difficult to
?isolate nucleic acids from them of a quality suitable for molecular analysis. 更看更多Standard preservation techniques
use formalin that maintains tissue structure and prevents putrefaction, but which also traps nucleic acids and modifies
?them through protein-protein and protein-nucleic acid crosslinks. RNA (and to some extent DNA) is often so fragmented
and chemically modified that it is incompatible with many molecular analysis techniques. RNA fragmentation in FFPE tissues
cannot be reversed; however, the protease digestion conditions of the RecoverAll? kit are designed to release a maximal
amount (see figure) of trapped RNA fragments of all sizes, including microRNA, in a relatively short amount of time.
The RecoverAll? Total Nucleic Acid Isolation Kit solution
The RecoverAll? Total Nucleic Acid Isolation Kit procedure requires about 45 minutes of hands-on time and can be
?completed in typically less than 1 day for RNA. FFPE samples are deparaffinized using a series of xylene and ethanol
?washes. Next, they are subjected to an extensive protease digestion with an incubation time tailored for the recovery
of either RNA or DNA. Nucleic acids are then purified using a rapid glass-filter method, and are eluted into either water
or low-salt buffer.
Nucleic acids for almost any downstream application
As is the case with all FFPE tissue, sample fixation and storage typically cause nucleic acid fragmentation and modification.
Therefore, downstream applications, such as microarray analysis, which require more pristine RNA than does qRT-PCR, may require
?modification for optimal results. Although DNA tends not to fragment as easily as RNA, it appears to be more reactive to the
formalin and requires a longer (2-day) protease digestion time to release substantial amounts of DNA.
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詳細(xì)說明
常用規(guī)格
| Sample Type (General): |
FFPE & Fixed Samples? |
| Final Product: |
Genomic DNA,? micro RNA,? Total RNA? |
| Downstream Application: |
Southern Blotting,? Microarray Analysis,? cDNA Library Construction,? PCR,? RT-PCR (Endpoint),? Northern Blotting,? Real-Time PCR,? microRNA Analysis? |
| High Throughput Compatibility: |
Manual Protocols,? Not High Throughput-Compatible? |
| Isolation Technology: |
Spin Column (Glass Fiber Filter)? |
| Product Size: |
40 purifications? |
| Yield (up to): |
24 μg? |
| Starting Material (Amount): |
Up to 35 mg of unsectioned core samples,? Up to four 20 μm sections? |
| Regulatory Statement: |
For Research Use Only. Not for use in diagnostic procedures.? |
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內(nèi)容和儲(chǔ)存
Store Digestion Buffer, Wash 1 Concentrate, Wash 2/3 Concentrate, Collection Tubes, Filter Cartridges, Isolation
Additive and Elution Solution at room temperature. Store Protease, 10X DNase Buffer, DNase and Rnase A at -20°C.