重組人CD138蛋白（Recombinant Human CD138 Protein），GMP級

產品貨號：GMP-TL685

產品描述（Description）

Syndecan-1 (SYND1 or SDC1) is also known as CD antigen CD138, is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 / SDC1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a useful marker for plasma cells, but only if the cells tested are already known to be derived from blood.

分子別名（Synonym）

SDC1、Syndecan-1、SYND1、SDC

表達宿主（Expression host）

HEK293細胞

來源（Source）

Human

蛋白序列（Protein Sequence）

DNA 序列編碼人 CD138（UniProtKB/Swiss-Prot: P18827.3）表達帶有 Fc 標簽在 C 末端

分子量（Molecular Weight） 

重組人 CD138 蛋白 包含 463 個氨基酸，預測理論分子量為 50.8kD

純度（Purity）

≥95%采用SDS-PAGE凝膠和高效液相色譜分析

內毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

以固定蛋白來增強 Saos2（人骨肉瘤細胞）對人纖維連接蛋白黏附的能力來檢測，將 5×10?細胞/孔 加入到人 Syndocan-1 和人纖維連接蛋白（0.5ug/ml）包被板中，細胞黏附以劑量依賴性方式增強。這種作用的 ED??為 5-10ug/ml

穩定性（Stability）

凍干的樣本的可以在4℃保存24個月，溶解后的液體可以于-20℃保存6-12個月，并且避免反復凍融

組成（Component）

含有6%甘露醇的無菌PBS（PH 7.4）的凍干粉

參考文獻

1、Syndecan-1 (CD138) Modulates Triple-Negative Breast Cancer Stem Cell Properties via Regulation of LRP-6 and IL-6-Mediated STAT3 Signaling Sherif A. Ibrahim, Hebatallah Hassan, Laura Vilardo, Sampath Katakam Kumar, Archana Vijaya Kumar, Reinhard Kelsch, Cornelia Schneider, Ludwig Kiesel, Hans Theodor Eich, Ileana Zucchi, Rolland Reinbold, Burkhard Greve, Martin G?tte PLoS One. 2013; 8(12): e85737. Published online 2013 Dec 31. doi: 10.1371/journal.pone.0085737 

2、Syndecan-1 (CD138) deficiency increases Staphylococcus aureus infection but has no effect on pathology in a mouse model of peritoneal dialysis Paulina M. Kowalewska, Uyen T. Nguyen, Lori L. Burrows, Alison E. Fox-Robichaud J Biomed Sci. 2016; 23: 20. Published online 2016 Feb 1. doi: 10.1186/s12929-016-0232-9

  注意：For research and manufacturing use.