KDR (phospho Y996) polyclonal antibody
產品名稱： KDR (phospho Y996) polyclonal antibody
英文名稱： KDR (phospho Y996) polyclonal antibody
產品編號： PAB0505
產品價格： null
產品產地： 臺灣
品牌商標： Abnova
更新時間： null
使用范圍：
亞諾法生技股份有限公司（Abnova)
- 聯系人 :
- 地址 : 臺灣臺北市內湖區洲子街 108 號 9 樓
- 郵編 : 11493
- 所在區域 : 臺灣
- 電話 : +886-920**1152 點擊查看
- 傳真 : 點擊查看
- 郵箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of KDR.
- Immunogen:
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y996 of human KDR.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Platelet factor 4 disrupts the intracellular signalling cascade induced by vascular endothelial growth factor by both KDR dependent and independent mechanisms.
Sulpice E, Contreres JO, Lacour J, Bryckaert M, Tobelem G.Eur J Biochem. 2004 Aug;271(16):3310-8.
- 2.
- Regulation of vascular endothelial growth factor receptor 2-mediated phosphorylation of focal adhesion kinase by heat shock protein 90 and Src kinase activities.
Le Boeuf F, Houle F, Huot J.J Biol Chem. 2004 Sep 10;279(37):39175-85. Epub 2004 Jul 6.
- 3.
- VEGF receptor phosphorylation status and apoptosis is modulated by a green tea component, epigallocatechin-3-gallate (EGCG), in B-cell chronic lymphocytic leukemia.
Lee YK, Bone ND, Strege AK, Shanafelt TD, Jelinek DF, Kay NE.Blood. 2004 Aug 1;104(3):788-94. Epub 2004 Mar 2.
- Applications
- Western Blot (Cell lysate)
- KDR (phospho Y996) polyclonal antibody (Cat # PAB0505) is used in Western blot to detect Phospho-KDR-Y996 in HMVEC cell lysate. Endothelial cells were stimulated with 50 ug/mL VEGF for 5min; 20 ug lysate from HMVEC was loaded onto an 8% gel; for Western blot, membranes were incubated O/N with KDR (phospho Y996) polyclonal antibody (Cat # PAB0505) diluted to 1 : 500 in 1% Milk/TBST. Data and Protocol kindly provided by Dr. Weis from Cheresh Lab, UCSD.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with KDR (phospho Y996) polyclonal antibody (Cat # PAB0505) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 3791
- Protein Accession#:
- NP_002244;P35968
- Gene Name:
- KDR
- Gene Alias:
- CD309,FLK1,VEGFR,VEGFR2
- Gene Description:
- kinase insert domain receptor (a type III receptor tyrosine kinase)
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq
- Other Designations:
- soluble VEGFR2,vascular endothelial growth factor receptor 2
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