LightCycler? Uracil-DNA Glycosylase??

雙雜交探針?尿嘧啶-DNA 糖基化酶

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For general laboratory use.

供一般實驗室使用。

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Product No：03539806001???產品編號：03539806001 ?

Pack Size：100 U (100 reactions)??包裝尺寸：100 U（100 次反應）

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Keywords: Other qPCR Consumables??關鍵詞：其他 qPCR 耗材

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Overview概述：

LightCycler? Uracil-DNA Glycosylase hydrolyzes uracil-glycosidic bonds at U-DNA sites in single- and double-stranded DNA, excising uracil and creating alkali- and heat-sensitive abasic sites in the DNA.

LightCycler ? 尿嘧啶-DNA糖基化酶水解單鏈和雙鏈DNA中U-DNA位點的尿嘧啶-糖苷鍵，切除尿嘧啶并在DNA中產生對堿和熱敏感的脫堿基位點。

The enzyme is more active on single-stranded DNA than on double- stranded DNA.

該酶對單鏈 DNA 的活性高于對雙鏈 DNA 的活性。

The enzyme is inactive on RNA and native, uracil-free DNA.

該酶對 RNA 和天然無尿嘧啶 DNA 無活性。

Contents?內容

The enzyme is supplied in storage buffer at 2 U/μl.

該酶以 2 U/μl 的濃度提供在儲存緩沖液中。

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Application應用：

LightCycler? Uracil-DNA Glycosylase (LightCycler? UNG) is ideally suited for preventing carryover contamination between PCRs. This technique involves incorporating deoxyuridine triphosphate (dUTP) into all amplification reactions, and pretreating all successive LightCycler? FastStart Enzyme-based PCR mixtures with LightCycler? UNG. Prior to the thermal-cycling procedure, LightCycler? UNG is added to the LightCycler? amplification reaction mixture, then incubated for 10 minutes at +40°C. During the initial activation of FastStart DNA Polymerase (+95°C for 10 minutes), the LightCycler? UNG becomes inactive.

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LightCycler ? 尿嘧啶-DNA 糖基化酶 (LightCycler ? ?UNG) 非常適合防止 PCR 之間的殘留污染。該技術包括將脫氧尿苷三磷酸 (dUTP) 加入所有擴增反應中，并使用 LightCycler ? ?UNG預處理所有連續的基于LightCycler ? ?FastStart 酶的 PCR 混合物。在熱循環程序之前，將 LightCycler ? ?UNG 添加到 LightCycler ? 擴增反應混合物中，然后在 +40°C 下孵育 10 分鐘。在 FastStart DNA 聚合酶的初始激活期間（+95°C 10 分鐘），LightCycler ? ?UNG 變得不活躍。

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Note: LightCycler? UNG is designed only for use with the FastStart Enzyme or FastStart Enzyme-based LightCycler? Kits.

注意： LightCycler? ?UNG 僅設計用于 FastStart 酶或基于 FastStart 酶的 LightCycler ? 試劑盒。