Anti-IDH1 (R132P) Mouse Monoclonal Antibody-抗體-抗體-生物在線
武漢紐斯特生物技術有限公司
Anti-IDH1 (R132P) Mouse Monoclonal Antibody

Anti-IDH1 (R132P) Mouse Monoclonal Antibody

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產品名稱： Anti-IDH1 (R132P) Mouse Monoclonal Antibody

英文名稱： Anti-IDH1 (R132P) Mouse Monoclonal Antibody

產品編號： 26406

產品價格： null

產品產地： 美國

品牌商標： NewEastBio

更新時間： null

使用范圍： ELISA、WB、 IHC

武漢紐斯特生物技術有限公司
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IDH1 (R132P) [26406]
[100ul]		 ?

Catalog Number:??26406
Gene Symbol:??IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH
Description: ??Anti-IDH1 (R132P) Mouse Monoclonal Antibody
Background: ??Isocitrate dehydrogenases (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.
Immunogen: ??A synthetic peptide from the internal region of IDH1 which includes the mutation of R132P, human origin.
Applications: ??ELISA, WB, IHC
Recommended Dilutions: ??
ELISA: 1:1000-1:5000?
WB: 1:100-1:1000?
IHC: 1:50-1:100
Concentration: ???1 mg/ml
Host: ??Mouse
Format: ??Liquid
Clonality: ??Monoclonal
Isotype: ??
Purity: ??Purified from ascites
Storage buffer:
Preservative:??no
Constituents:??PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:??recognizes R132P mutant, but not wild-type IDH2 of vertebrates.
Storage Conditions:???Store at -20°C. Avoid freeze / thaw cycles
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Western blot:??
Western blot analysis of recombinant IDH1 (R132P) and wildtype proteins. Purified His-tagged IDH1 (R132P) (lane 1) and corresponding wild-type IDH1 protein (lane 2) were blotted with anti-IDH1 (R132P) monoclonal antibody (Cat. #26406).
Immunofluorescence:??
Immunofluorescence of cells expressing IDH1 proteins with anti-IDH1 (R132P) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH1(R132P) plasmid, pCDNA3-GFP-IDH1 (WT) plasmid , pCDNA3-GFP-IDH1(R132S) plasmid, pCDNA3-GFP-IDH1(R132L) plasmid , pCDNA3-GFP-IDH1(R132G) plasmid or pCDNA3-GFP-IDH1(R132C) plasmid , then fixed and stained with anti-IDH1 (R132P) monoclonal antibody (Cat. #26406).
Publications:
1.??Specific monoclonal antibodies against IDH1/2 mutations as diagnostic tools for gliomas
????Brain Tumor Pathology. January 2015, Volume 32, Issue 1, pp 3-11
2.??Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer
????Nature 513, 110–114 (04 September 2014)
3.??Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism
????Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015

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