Active人ADAM12蛋白片段
產(chǎn)品名稱： Active人ADAM12蛋白片段
英文名稱： A disintegrin and metalloproteinase domain 12 ADA12_HUMAN ADAM 12 ADAM metallopeptidase domain 12 ADAM12 Disintegrin and metalloproteinase d
產(chǎn)品編號(hào)： ab174014
產(chǎn)品價(jià)格： 0
產(chǎn)品產(chǎn)地： 英國(guó)
品牌商標(biāo)： abcam
更新時(shí)間： null
使用范圍： null
- 聯(lián)系人 :
- 地址 : 深圳市寶安區(qū)西鄉(xiāng)寶民二路賢基大廈4E
- 郵編 :
- 所在區(qū)域 : 廣東
- 電話 : 133****4454 點(diǎn)擊查看
- 傳真 : 點(diǎn)擊查看
- 郵箱 : 1484332550@qq.com
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- 性質(zhì)Recombinant
- 來(lái)源HEK 293 cells
- 氨基酸序列
- AccessionO43184-4
- 種屬Human
- 序列RGVSLWNQGRADEVVSASVGSGDLWIPVKSFDSKNHPEVLNIRLQRESKE LIINLERNEGLIASSFTETHYLQDGTDVSLARNYTGHCYYHGHVRGYSDS AVSLSTCSGLRGLIVFENESYVLEPMKSATNRYKLFPAKKLKSVRGSCGS HHNTPNLAAKNVFPPPSQTWARRHKRETLKATKYVELVIVADNREFQRQG KDLEKVKQRLIEIANHVDKFYRPLNIRIVLVGVEVWNDMDKCSVSQDPFT SLHEFLDWRKMKLLPRKSHDNAQLVSGVYFQGTTIGMAPIMSMCTADQSG GIVMDHSDNPLGAAVTLAHELGHNFGMNHDTLDRGCSCQMAVEKGGCIMN ASTGYPFPMVFSSCSRKDLETSLEKGMGVCLFNLPEVRESFGGQKCGNRF VEEGEECDCGEPEECMNRCCNATTCTLKPDAVCAHGLCCEDCQLKPAGTA CRDSSNSCDLPEFCTGASPHCPANVYLHDGHSCQD
- 分子量55 kDa including tags
- 氨基酸29 to 513
- 標(biāo)簽His tag C-Terminus
技術(shù)指標(biāo)
Our?Abpromise guarantee?covers the use of?ab174014?in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 生物活性
Measured by its ability to cleave IGFBP-3. The bioactivity was measured in 100 μL reaction mixture containing 20 μg/ml of ab174014 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5.
- 應(yīng)用
SDS-PAGE
Functional Studies
- 內(nèi)毒素水平< 1.000?Eu/μg
- 純度> 98 % by SDS-PAGE.
- 形式Lyophilised
-
濃度由溶解方法決定?
制備和貯存
- 穩(wěn)定性和存儲(chǔ)
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C long term. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- 復(fù)溶It is recommended to reconstitute the lyophilized protein in 50 μl sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage
常規(guī)信息
-
別名
- A disintegrin and metalloproteinase domain 12
- ADA12_HUMAN
- ADAM 12
see all - 功能Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
- 組織特異性Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.
- 序列相似性Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain. - 結(jié)構(gòu)域The cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. - 翻譯后修飾The precursor is cleaved by a furin endopeptidase.
- 細(xì)胞定位Secreted and Cell membrane.
- Information by UniProt
Active human ADAM12 protein fragment 圖像
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SDS-PAGE - Active human ADAM12 protein fragment (ab174014)