女人与狗锁死了视频大全|霍金死亡过程恐怖视频|无节社团简谱季免费观看|六间房直播大厅|中文字幕欧美另类精品|久久人人玩人妻潮喷内射人人|亚洲国产一区二区三区在线观看

• 產品名稱11，12 DHET ELISA試劑盒
• 檢測方法Colorimetric
• 樣品類型
  Urine, Serum, Plasma, Cell culture extracts, Tissue
• 檢測類型Competitive
• 靈敏度
  10 pg/ml
• 范圍
  0.01 ng/ml - 1000 ng/ml
• 實驗步驟Multiple steps standard assay
• 產品概述

  Abcam’s?11,12 DHET?in vitro?competitive ELISA?kit is designed for accurate quantitative measurement in serum, plasma, urine, cell culture and?tissue samples following proper isolation and purification.

  ?

  This competitive ELISA kit is based on competition between the 11,12 DHET epitope and the 11,12 DHET-HRP conjugate for a limited number of binding sites available from the anti-11,12 DHET antibody; which is coated to the wells of the 96 well ELISA plate. The conjugate concentration is held as a constant in each well, while the concentration of the 11,12 DHET is variable, based on the concentration of the sample or standard. Thus the amount of the 11,12 DHET conjugate which is able to bind to each of the wells is inversely proportional to the concentration of 11,12 DHETin the standard or sample. The amount of the conjugate which is bound to each well is then determined by the amount of color obtained, when TMB is added. The TMB reacts with the HRP available in the well. With the addition of sulfuric acid, the blue colored product is converted into a yellow colored product, which can be read on a plate reader at 450 nm.

  The level of 11,12-DHET or 11,12-DHET epitope has been shown to exhibit correlation with hypertension in rodents (1,2,3). 11,12-DHET is a representative metabolite of cytosolic epoxide hydrolase-mediated metabolism of EETs, which are generated by arachidonic acid epoxygenase activity of cytochrome P450’s (4).

• 經測試應用Competitive ELISAmore details
• 平臺Microplate

性能

• 存放說明Store at -20°C. Please refer to protocols.

• 組件	1 x 96 tests
  11,12-DHET Standard	1 x 2μl
  11,12-DHET-HRP Conjugates	1 x 12μl
  HRP Buffer	1 x 15ml
  Pre-coated 96-Well Microplate	1 unit
  Sample Dilution Buffer	1 x 25ml
  TMB Substrate	1 x 24ml
  Wash Buffer Solution	1 x 25ml

• 研究領域
  • Signal Transduction
  ?
  • Signaling Pathway
  ?
  • Lipid Signaling
  ?
  • Other

  • Signal Transduction
  ?
  • Metabolism
  ?
  • Lipid metabolism

  • Metabolism
  ?
  • Pathways and Processes
  ?
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  ?
  • Fatty acids

應用

11,12 DHET ELISA Kit 圖像

• 

  Typical Standard Curve

  Representative standard curve using ab175814 11,12 DHET ELISA Kit.