從細胞膜、胞漿到細胞核，存在多條信號通路串聯交叉形成的復雜信號網絡。該信號網絡在細胞受到胞外刺激后將信號通過級聯放大、分散調節等方式傳入胞內，引起一系列的綜合性細胞應答。一種生物效應的出現往往存在多條信號通路的同步活化，可逆的磷酸化修飾反應則是細胞內部最為普遍和節能的信號蛋白活化調節方式。因此，找到激活的信號通路乃至發生磷酸化調變的通路蛋白，往往成生命科學研究的起點。 ? ? ? ? ? ? ? ?

信號通路磷酸化廣譜篩選抗體芯片（PEX100），采用三維高分子膜專利技術，在片基上高密度結合??1318??種高特異抗體，分別檢測432個信號蛋白的679個磷酸化位點。這些信號蛋白廣泛參與多條重要信號通路信號傳導過程。芯片針對每一個特定蛋白磷酸化位點，設置一對抗體分別檢測其磷酸化（Phospho）和非磷酸化（non-Phospho）狀態以提高磷酸化檢測靈敏度和穩定性。一次芯片實驗即可實現多條信號通路的同步篩選和具體調變位點的清晰定位，為后續生物現象的深入探索提供明確的研究方向。 ? ? ? ? ? ? ? ?

?

芯片特點： ? ? ? ? ? ? ? ?

• 1. 芯片規格為76 x 25 x 1 mm；

• 2. 實現31條信號通路全面篩選；

• 3. 每種抗體設置2次技術重復；

• 4. 適用于組織、細胞等多類型樣本；

• 5. 5x10⁶細胞、200μg總蛋白量即可滿足實驗；

• 6. 每個檢測位點設有磷酸化和非磷酸化配對抗體；

• 7. 可通用于人、小鼠、大鼠等多類型模式生物檢測。

?檢測原理：?? ? ? ? ? ? ? ?

? ? ? ? ? ? ? ?

?

?? ? ? ? ? ? ? ?

?

檢測列表： ? ? ? ? ? ? ? ?

?? ? ? ? ? ?詳細列表下載? ? ? ? ? ? ? ??

?

華盈生物還提供針對目標信號通路研究的蛋白磷酸化抗體芯片：

?

?

詳情請點擊http://www.wayenbio.com/11-205057-75185.html進行查詢。

?

?相關文獻：?? ? ? ? ? ? ? ?

1. Luo L L, Zhao L, Wang Y X, et al.?Insulin-like growth factor binding protein-3 is a new predictor of radiosensitivity on esophageal squamous cell carcinoma.?Sci Rep-UK, 2015, 5 ? ?(中山大學附屬腫瘤醫院)

2. Zhu Y P, Wan F N, Shen Y J, et al.?Reactive stroma component COL6A1 is upregulated in castration-resistant prostate cancer and promotes tumor growth.?Oncotarget,?2015, 6(16): 14488. ? ?（復旦大學附屬腫瘤醫院）

3. Wang T, Han S, Wu Z, et al.?XCR1 promotes cell growth and migration and is correlated with bone metastasis in non-small cell lung cancer.?Biochem Bioph Res Co, 2015, 464(2): 635-641. ? （上海長征醫院）

4. Chen P, Huang H, Wu J, et al.?Bone marrow stromal cells protect acute myeloid leukemia cells from anti‐CD44 therapy partly through regulating PI3K/Akt–p27Kip1 axis.?Mol Carcinogen, 2015 Dec;54(12):1678-85. ? ? (福建醫科大學附屬協和醫院)

5. Wan F, et al.?Oxidized low-density lipoprotein is associated with advanced-stage prostate cancer.?Tumor Biol, 2015: 1-10. ? ?(復旦大學附屬腫瘤醫院)

6. Zhu R, et al.?pH sensitive nano layered double hydroxides reduce the hematotoxicity and enhance the anticancer efficacy of etoposide on non-small cell lung cancer.Acta Biomater. 2016 Jan 1;29:320-32. ? ?(同濟大學)

7. Li W, et al.?Withaferin A suppresses the up-regulation of acetyl-coA carboxylase 1 and skin tumor formation in a skin carcinogenesis mouse model.Mol Carcinog. 2015 Oct 16. ? ?(河北大學)

8. Eke I, et al.?Simultaneous β1 integrin-EGFR targeting and radiosensitization of human head and neck cancer.?J Natl Cancer I, 2015, 107(2): dju419.

9. Lund R R, et al.?NADH-cytochrome b5 reductase 3 promotes colonization and metastasis formation and is a prognostic marker of disease-free and overall survival in estrogen receptor-negative breast cancer.?Mol Cell Proteomics, 2015, 14(11): 2988-2999.

10. Han B, et al.?The role of TGFBI (βig-H3) in gastrointestinal tract tumorigenesis.?Mol Cancer,2015, 14(1): 64.

11. Rao?W,?et?al.?OVA66?increases?cell?growth,?invasion?and?survival?via?regulation?of?IGF-1R-MAPK?signaling?in?human?cancer?cells.?Carcinogenesis,??April?7,?2014.（上海交通大學醫學院）

12. Jia D, et al.?Amplification of MPZL1/PZR promotes tumor cell migration through Src-mediated phosphorylation of cortactin in hepatocellular carcinoma.?Cell Res,?2014, (24):204-217. (上海仁濟醫院)?

13. Ding Y, et al.?Seed-targeting anti-miR-21 inhibiting malignant progression of retinoblastoma and analysis of their phosphorylation signaling pathways.?Exp Eye Res, 2014. (暨南大學)?

14. Dong S, et al.?The REGγ Proteasome Regulates Hepatic Lipid Metabolism through Inhibition of Autophagy.?Cell Metab, 2013, 18(3): 380-391. （華東師范大學）

15. Li L et al.?REGγ deficiency promotes premature aging via the casein kinase 1 pathway.Proc Natl Acad Sci USA.2013; June 13. （華東師范大學）

16. Cui WY et al.?Identification and Characterization of Poly(I:C)-induced Molecular Responses Attenuated by Nicotine in Mouse Macrophages.?Mol Pharmacol.?2013; 83:61-72. （浙江大學）

17. Eke I et al.?EGFR/JIP-4/JNK2 signaling attenuates Cetuximab-mediated radiosensitization of squamous cell carcinoma cells.?Cancer Research. 2013; 73(1):297-306.

18. Totonchy JE et al.?Aberrant Proliferation in CXCR7+ Endothelial Cells via Degradation of the Retinoblastoma Protein.?PLoS ONE.?2013; 8(7): e69828.

19. Xu N et al.?Activation of RAW264.7 mouse macrophage cells in vitro through treatment with recombinant ricin toxin-binding subunit B: Involvement of protein tyrosine, NF-κB and JAK-STAT kinase signaling pathways.?Int J Mol Med. 2013; 32(3): 729-735. （吉林大學）

20. Li F et al.?Superoxide Mediates Direct Current Electric Field-Induced Directional Migration of Glioma Cells through the Activation of AKT and ERK.?PLoS ONE.?2013; 8(4): e61195. （第三軍醫大學）

21. Ranzato E et al.?Epithelial mesenchymal transition traits in honey-driven keratinocyte wound healing: comparison among different honeys.?Wound Repair Regen.?2012; 20(5):778-85.

22. Fan H et al.?Cardioprotective Effects of Salvianolic Acid A on Myocardial Ischemia-Reperfusion Injury In Vivo and In Vitro.?Evid Based Complement Alternat Med. 2012; 508938. （煙臺大學）

23. Zhang YM et al.?A novel angiogenesis inhibitor impairs lovo cell survival via targeting against human VEGFR and its signaling pathway of phosphorylation.?Cell Death Dis. 2012; 3: e406. （西安交通大學）

24. Li Hui et al.?Increased prevalence of regulatory T cells in the lung cancer microenvironment: a role of thymic stromal lymphopoietin.?Cancer Immunology Immunotherapy.?2011; 60(11): 1587-1596. （天津醫科大學）

25. Bernier M et al.?Negative regulation of STAT3 protein-mediated cellular respiration by SIRT1 protein. J Biol Chem.?2011; 286(22): 19270-19279.

26. Kang S et al.?p90 ribosomal S6 kinase 2 promotes invasion and metastasis of human head and neck squamous cell carcinoma.?J Clin Invest?. 2010; 120(4):1165-1177.

?