ACE (CD143)小鼠ELISA試劑盒
產品名稱: ACE (CD143)小鼠ELISA試劑盒
英文名稱: ACE (CD143)小鼠ELISA試劑盒(ab155452)
產品編號: ab155452
產品價格: null
產品產地: 英國
品牌商標: abcam
更新時間: null
使用范圍:
- 聯系人 :
- 地址 : 深圳市寶安區西鄉寶民二路賢基大廈4E
- 郵編 :
- 所在區域 : 廣東
- 電話 : 133****4454 點擊查看
- 傳真 : 點擊查看
- 郵箱 : 1484332550@qq.com
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- 檢測方法Colorimetric
- 精確度
批次內 樣品 n Mean SD CV% overall ? ? ? < 10% ? - 樣品類型
Cell culture supernatant, Serum, Plasma
- 檢測類型Sandwich (quantitative)
- 靈敏度
< 0.12 ng/ml
- 范圍
30 ng/ml - 0.123 ng/ml
- 復檢
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特定樣本復檢結果 樣品類型 平均% 范圍 Serum 104.6 96% - 112% Plasma 101.4 94% - 109% Cell culture media 99.78 90% - 109% ?
- 實驗步驟Multiple steps standard assay
- 種屬反應性
與反應:?Mouse
- 產品概述
Abcam's ACE (CD143) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an?in vitro?enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse ACE (CD143) in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse ACE coated on a 96-well plate. Standards and samples are pipetted into the wells and ACE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse ACE antibody is added. After washing away unbound biotinylated antibody,
HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ACE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- 經測試應用Sandwich ELISAmore details
- 平臺Microplate
性能
- 存放說明Store at -20°C. Please refer to protocols.
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組件 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200μl ACE Microplate (12 x 8 wells) 1 unit Assay Diluent A 1 x 30ml Biotinylated anti-mouse ACE 2 vials Recombinant Mouse ACE Standard 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml - 研究領域
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