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Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco? MEM modifications for a range of cell culture applications. Find the right formulation using the?media selector tool.

The complete?formulation?is available.

Gibco? MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM?is available with Earle’s salts for use in a CO2?incubator, or with Hank’s salts for use without CO2. This product is made with Earle’s salts.

Product Intended Use
For?in vitro?diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
For supply chain continuity, Life Technologies manufactures Gibco? MEM at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers. In addition, the New York facility has ISO 9001 certification.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10%?Fetal Bovine Serum?(FBS). MEM uses a sodium bicarbonate buffer system (2.2 g / L) and therefore requires a 5-10% CO2?environment to maintain physiological pH. Powder forms of Gibco? cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see?protocol?for details).

內容和儲存

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Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture

詳細說明

常用規格