?英文名稱NSE
中文名稱神經元特異性烯醇化酶單克隆抗體
別? ? 名Gamma-enolase; Gamma enolase; Neural enolase; Neuron specific enolase; neurone-specific enolase; 2 phospho D glycerate hydrolyase; Eno 2; Eno2; ENO2; ENOG; Enolase 2; Enolase 2 gamma neuronal; Enolase2; Neuron specific enolase; Neuron specific gamma enolase; NSE; ENOG_HUMAN; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; enolase 2, gamma, neuronal.??
研究領域腫瘤? 細胞生物? 免疫學? 神經生物學??
抗體來源Mouse
克隆類型Monoclonal
克 隆 號15E2
交叉反應Human, Mouse, Rat,?
產品應用WB=1:500-2000 IHC-P=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量48kDa
細胞定位細胞漿 細胞膜?
性? ? 狀Liquid
濃? ? 度1mg/ml
免 疫 原Recombinant human NSE protein:?
亞? ? 型IgG
純化方法affinity purified by Protein G
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產品介紹This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008].
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Function:
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
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Subunit:
Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific.
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Subcellular Location:
Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
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Tissue Specificity:
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
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Similarity:
Belongs to the enolase family.
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SWISS:
P09104
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Gene ID:
2026
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Database links:
Entrez Gene: 2026 Human
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Entrez Gene: 13807 Mouse
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Entrez Gene: 24334 Rat
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Omim: 131360 Human
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SwissProt: P09104 Human
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SwissProt: P17183 Mouse
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SwissProt: P07323 Rat
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Unigene: 511915 Human
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Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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神經元特異性烯醇化酶（NSE）存在于神經元細胞和神經內分泌組織中，起源于神經內分泌細胞的腫瘤可產生過量的NSE。NSE也是小細胞肺癌的檢測指標，70％左右的小細胞肺癌患者血中NSE升高，而其他組織型肺癌NSE升高的患者僅為10％～20％。
產品圖片
Sample: A431 Cell (Human) Lysate at 40 ug
Primary: Anti- NSE (bsm-33072M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD
Sample:
SH-SY5Y (Human) Cell Lysate at 30 ug
U87MG (Human) Cell Lysate at 30 ug
Primary: Anti-NSE (bsm-33072M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NSE) Monoclonal Antibody, Unconjugated (ascites of bsm-33072M 15E2) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NSE) Monoclonal Antibody, Unconjugated (ascites of bsm-33072M 15E2) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
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