Anti-Epithelial Keratin Type II, clone AE-3
Cat. No: MC-976 (250 μg)
Background: This antibody recognizes most
of the basic (type II) keratins. Each epithelium
contains at least one basic keratin. AE-3 is
broadly reactive with almost all epithelial and
their neoplasms. This antibody together with
MC-975 (clone AE-1) has been used for
studying the immuno-localization of epidermal
keratins, tissue distribution and epithelial
development. AE-1 and AE-3 have also been
used extensively, individually or mixed, for
distinguishing carcinoma from non-carcinoma.
Specificity: AE-3 detects distinct MW’s of 52
kDa, 54 kDa, 56 kDa, 58 kDa, 59 kDa, 64 kDa,
and 65-67 kDa, with all Ip’s lower than 6.0.
These MW’s are believe to correspond with
keratin 8, 7, 6, 5, 4, 3 and 1&2, respectively.
Species Reactivity: Human, bovine, chicken,
mouse, rabbit, and rat. Others not tested.
Ig Isotype: Mouse IgG1
Format: 250 μg purified IgG at 1 mg/ml with
0.1 sodium azide.
Storage and Stability: Store undiluted
antibody at 2 - 8°C. For long term, aliquot and
store at -20°C. Avoid freeze/thaw cycles.
Applications and Suggested Dilutions:

Immunohistochemistry: (Formalin-fixed /
Paraffin-embedded sections) Use at 1:150-
1:200 for 30 min. at 37°C. Higher dilutions
can be used if sections are incubated at 4°C
overnight.

Western blot: Use at 1:100.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.